MUTANT MOUSE RESOURCE AND RESEARCH CENTER: AIDS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 关于艾滋病 描述(由申请人提供)： 基因工程小鼠(GEM)作为人类健康和疾病模型的重要性已被明确证明。由于宝石在生物医学研究中的重要性，宝石线的数量在过去五年中呈指数级增长；预计这种天文数字增长将继续下去。认识到在研究人员之间共享老鼠的科学重要性，NIH制定了一项老鼠共享政策，该政策规定，在NIH资助下产生的小鼠品系必须分发并与科学界共享。为了实现这一目标，国立卫生研究院在1999年和2000年资助建立了四个区域突变小鼠资源和研究中心(MMRRC)。这项申请是对密苏里州/哈兰(M/H)MMRRC的一次竞争性续签。该提案的具体目标是：(1)提高MMRRC服务职能的效率，同时继续为科学界提供最高质量的突变鼠库和配送中心。为此，我们将采用最先进的冷冻保存和复活技术，例如卵胞浆内单精子注射(ICSI)、核移植(NT)卵巢组织(OT)移植(OTT)和将NT来源的胚胎干细胞(NTES细胞)注入四倍体胚胎(NTES/4N)。使用这些方法，我们将能够更有效地导入、冷冻保存和复活胚胎，而成本大约是目前基于传统胚胎冷冻保存方法的10%。(2)进行研究，以增加资源中心的价值。研究将包括旨在改进用于小鼠品系复活的辅助生殖技术(ARTS)的项目，开发用于检测病原体和突变小鼠群体健康监测的生物传感器，以及开发新的遗传和ARTS，以彻底提高制造同种小鼠品系的速度和效率。所提出的M/H MMRRC操作方法代表了小鼠资料库的一种新范式。认识到创业板的持续指数增长，新的范式是强制性的；现有的鼠标资源中心根本无法使用传统方法将所有重要的鼠标系列存档。此外，拟议的研究项目将为MMRRC带来更高的效率，并开发对整个科学界有用的新技术。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Regarding AIDS DESCRIPTION (provided by applicant): The importance of genetically-engineered mice (GEM) as models of human health and disease has been demonstrated unequivocally. Because of the importance of GEM in biomedical research, the numbers of GEM lines have grown exponentially over the last five years; this astronomical growth is predicted to continue. Recognizing the scientific importance of sharing mice among investigators, the NIH developed a mouse sharing policy which states that mouse lines generated with the aid of NIH funding must be distributed and shared with the scientific community. To accomplish this goal, the NIH funded the establishment of four regional Mutant Mouse Resource and Research Centers (MMRRCs) in 1999 and 2000. This application is a competitive renewal for the Missouri/Harlan (M/H) MMRRC. Specific Aims of the proposal are: (1) To improve the efficiencies of the MMRRC service functions while continuing to provide the scientific community with a mutant mouse repository and distribution center of the highest quality. To accomplish this, we will employ state-of-the-art cryopreservation and reanimation (reestablishment of lines after cryopreservation) technologies, such as intracytoplasmic sperm injection (ICSI), nuclear transfer (NT) ovarian tissue (OT) transplantation (OTT) and injection of NT derived embryonic stem cells (ntES cells) into tetraploid embryos (ntES/4N). Using these approaches we will be able to more efficiently import, cryopreserve and reanimate lines at a cost approximately 10% of current, traditional embryo cryopreservation based approaches. (2) To conduct research aimed at increasing the value of the MMRRC. Research will include projects aimed at improving assisted reproductive technologies (ARTs) used for reanimation of mouse lines, development of biosensors for detection of pathogens and health surveillance of mutant mouse colonies, and development of new genetic and ARTs that will revolutionize the speed and efficiency with which congenic strains of mice are made. The proposed approach to the operation of the M/H MMRRC represents a new paradigm for mouse repositories. Recognizing the continued exponential growth of GEM, a new paradigm is mandatory; existing mouse resource centers simply cannot archive all of the important mouse lines using traditional methodology. Furthermore, the proposed research projects will result in even greater efficiencies for the MMRRC and the development of new technologies useful to the entire scientific community.