MUTANT MOUSE RESOURCE AND RESEARCH CENTER: ANIMAL EMBRYONIC STEM CELLS

突变小鼠资源与研究中心：动物胚胎干细胞

• 批准号: 7360415
• 负责人: JOHN K. CRITSER
• 金额: $ 19.25万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7360415
• 关键词: MUTANT; MOUSE; RESOURCE; RESEARCH; CENTER

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The importance of genetically-engineered mice (GEM) as models of human health and disease has been demonstrated unequivocally. Because of the importance of GEM in biomedical research, the numbers of GEM lines have grown exponentially over the last five years; this astronomical growth is predicted to continue. Recognizing the scientific importance of sharing mice among investigators, the NIH developed a mouse sharing policy which states that mouse lines generated with the aid of NIH funding must be distributed and shared with the scientific community. To accomplish this goal, the NIH funded the establishment of four regional Mutant Mouse Resource and Research Centers (MMRRCs) in 1999 and 2000. This application is a competitive renewal for the Missouri/Harlan (M/H) MMRRC. Specific Aims of the proposal are: (1) To improve the efficiencies of the MMRRC service functions while continuing to provide the scientific community with a mutant mouse repository and distribution center of the highest quality. To accomplish this, we will employ state-of-the-art cryopreservation and reanimation (reestablishment of lines after cryopreservation) technologies, such as intracytoplasmic sperm injection (ICSI), nuclear transfer (NT) ovarian tissue (OT) transplantation (OTT) and injection of NT derived embryonic stem cells (ntES cells) into tetraploid embryos (ntES/4N). Using these approaches we will be able to more efficiently import, cryopreserve and reanimate lines at a cost approximately 10% of current, traditional embryo cryopreservation based approaches. (2) To conduct research aimed at increasing the value of the MMRRC. Research will include projects aimed at improving assisted reproductive technologies (ARTs) used for reanimation of mouse lines, development of biosensors for detection of pathogens and health surveillance of mutant mouse colonies, and development of new genetic and ARTs that will revolutionize the speed and efficiency with which congenic strains of mice are made. The proposed approach to the operation of the M/H MMRRC represents a new paradigm for mouse repositories. Recognizing the continued exponential growth of GEM, a new paradigm is mandatory; existing mouse resource centers simply cannot archive all of the important mouse lines using traditional methodology. Furthermore, the proposed research projects will result in even greater efficiencies for the MMRRC and the development of new technologies useful to the entire scientific community.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。描述（由申请人提供）：基因工程小鼠（GEM）作为人类健康和疾病模型的重要性已得到明确证明。由于GEM在生物医学研究中的重要性，在过去五年中，GEM品系的数量呈指数级增长；这种天文数字般的增长预计还将继续。认识到在研究人员之间共享小鼠的科学重要性，NIH制定了一项小鼠共享政策，规定在NIH资助下产生的小鼠系必须与科学界共享。为了实现这一目标，NIH在1999年和2000年资助建立了四个区域性突变小鼠资源和研究中心（mmrrc）。该申请是密苏里/哈兰（M/H） MMRRC的竞争性更新。该提案的具体目标是：(1)提高MMRRC服务功能的效率，同时继续为科学界提供最高质量的突变小鼠储存库和分配中心。为了实现这一目标，我们将采用最先进的冷冻保存和再生（冷冻保存后的细胞系重建）技术，如卵胞浆内单精子注射（ICSI）、核移植（NT）卵巢组织移植（OTT）和将NT衍生的胚胎干细胞（ntES细胞）注射到四倍体胚胎（ntES/4N）中。使用这些方法，我们将能够更有效地导入、冷冻保存和复活细胞系，成本约为目前传统胚胎冷冻保存方法的10%。(2)开展旨在提高MMRRC价值的研究。研究将包括旨在改进辅助生殖技术（辅助生殖技术）的项目，这些技术用于小鼠品系的再生，开发用于检测病原体和监测突变小鼠菌落的健康的生物传感器，以及开发新的遗传和辅助生殖技术，以彻底改变小鼠同源品系的制造速度和效率。所提出的M/H MMRRC操作方法代表了一个新的鼠标存储库范例。认识到创业板的持续指数增长，一个新的范式是强制性的；现有的鼠标资源中心根本无法使用传统方法存档所有重要的鼠标行。此外，拟议的研究项目将为MMRRC带来更高的效率，并开发对整个科学界有用的新技术。