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Surface Nanostructures For The Study of Biomolecular Recognition,Chirality And Crystal Growth

用于生物分子识别、手性和晶体生长研究的表面纳米结构

基本信息

批准号：
EP/F025688/1
负责人：
Daniel Frankel
金额：
$ 38.08万
依托单位：
Newcastle University
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2007
资助国家：
英国
起止时间：
2007 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=EP%2FF025688%2F1
关键词：
Surface Nanostructures Study Biomolecular Recognition

项目摘要

The aim of this proposal is to use two dimensional ordered structures created under ultra high vacuum (UHV) conditions as a template for crystal growth in solution. These highly ordered nanostructures will be used to exploit molecular recognition and select desired molecules from solution. For example chiral surfaces will be created in order to exact enantiomeric discrimination from a racemic mixture. This will be achieved by evaporating small biomolecules, either nucleic acid bases or chiral amino acids, onto a gold single crystal surface. Such a surface will have a high level of molecular order as indicated by surface crystallography. The UHV evaporation technique will afford a high degree of control with regards to surface coverage, periodicity and orientation that could not be achieved via liquid deposition. Due to the inert characteristics of gold it will be possible to take out these surfaces from the UHV chamber and insert them into solution. Monolayers of the nucleic acid bases will also be produced on gold and the molecular recognition/selectivity from a solution of mixed bases will be measured. It is also envisaged that such well ordered monolayers could act as templates for three dimensional crystal growth from solution. Thus epitaxy, molecular recognition and selectivity will be examined using electrochemical scanning tunnelling microscopy (STM), scanning tunnelling spectroscopy and atomic force microscopy.

该提案的目的是使用在超高真空（UHV）条件下产生的二维有序结构作为溶液中晶体生长的模板。这些高度有序的纳米结构将用于开发分子识别和从溶液中选择所需的分子。例如，将产生手性表面，以便从外消旋混合物中精确区分对映异构体。这将通过将小的生物分子（核酸碱基或手性氨基酸）蒸发到金单晶表面上来实现。这样的表面将具有高水平的分子有序性，如表面晶体学所示。UHV蒸发技术将提供关于表面覆盖度、周期性和取向的高度控制，这是通过液相沉积无法实现的。由于金的惰性特性，将有可能从UHV室中取出这些表面并将其插入溶液中。还将在金上产生核酸碱基的单层，并将测量来自混合碱基溶液的分子识别/选择性。还设想这种有序的单层可以作为从溶液中三维晶体生长的模板。因此，外延，分子识别和选择性将使用电化学扫描隧道显微镜（STM），扫描隧道光谱和原子力显微镜检查。