IRF6 PATHWAYS AND GENE TARGETS IN PALATE DEVELOPMENT

味觉发育中的 IRF6 通路和基因靶标

• 批准号: 7479131
• 负责人: BRIAN C SCHUTTE
• 金额: $ 38.28万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7479131
• 关键词: Address; Alleles; Cells; Cleaved cell; Clinical; Complex; Critical Pathways; Development; Disease; Epithelium; Etiology; Fluorescence Microscopy; Gene Activation; Gene Expression; Gene Targeting; Genes; Genetic Models; Genotype; Goals; Histocytochemistry; Human; Interferons; Knockout Mice; Knowledge; Link; Location; Measures; Medial; Mediating; Mediator of activation protein; Methods; Microarray Analysis; Modeling; Molecular; Molecular Profiling; Mus; Mutant Strains Mice; Mutation; Palate; Pathway interactions; Phenotype; Population; Preventive Intervention; Process; RNA; Signal Pathway; Signal Transduction; Sorting - Cell Movement; Testing; Time; Transcriptional Activation; Transgenic Mice; Van der Woude syndrome; cell type; cleft lip and palate; craniofacial; mouse model; orofacial; palatal shelves; research study; transcription factor

The goal of Project 0003 is to discover the genes that contribute to isolated cleft lip and palate, a common disorder with complex etiology. This project will study IRF6, a gene that encodes the transcription factor Interferon Regulatory Factor 6. Mutations in IRF6 cause Van der Woude syndrome (VWS). VWS is an outstanding clinical model for isolated cleft lip and palate because the phenotypes of VWS and isolated cleft lip and palate are similar. It is also an outstanding genetic model, since alleles of IRF6 are highly associated with isolated cleft lip and palate in nine geographically distinct populations. These results prove that IRF6 is a key component of a critical pathway in palate development. In this proposal we use mouse models to identify the IRF6 pathway and other genes therein. There are two main hypotheses, 1) IRF6 is a mediator of Tgfb signaling and 2) IRF6 gene targets are essential for palate development. There are three Specific Aims. Specific Aim 1 will determine which cell-type expresses Irf6 in the palate. This basic information will immediately suggest Irf6 pathways and gene targets. Specific Aim 2 will determine which pathways require Irf6 for palate development. The Tgfb3 signaling pathway is essential for palate development and Tgfb3 is expressed at about the same time and place as Irf6. Knockout mice will be used to test the hypotheses that Tgfb3 is essential for expression of Irf6 in the palate shelves. Specific Aim 3 will use microarray analysis to identify the target genes for Irf6 during palate development. In addition, if Irf6 is a mediator for Tgfb3, then gene expression in the palate will be similar in Tgfb3-/- and Irf6-/- mice. Addressing these aims will advance our understanding of Irf6, a gene that is essential in craniofacial development. Further, the IRF6 pathways and targets, discovered in this proposal, will be assessed for their involvement in cleft lip and palate in Project 0001 and in palate development in Project 0004 and Cores 9003 and 9004. This aggregate knowledge will enhance our ability to determine the causes of, and develop preventive interventions for, orofacial clefts.