Flow Cytometry

流式细胞仪

• 批准号: 7697011
• 负责人: Allan J Zajac
• 金额: $ 7.14万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-16 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7697011
• 关键词: Acquired Immunodeficiency Syndrome; Address; Arts; Budgets; Cell Separation; Cell physiology; Cells; Color; Communities; Consultations; Coupled; Development; Ensure; Equipment; Flow Cytometry; Goals; HIV; Immune system; Immunity; Individual; Internet; Investigation; Lasers; Measures; Operative Surgical Procedures; Phenotype; Population; Research; Research Activity; Research Personnel; Resources; Services; Techniques; Technology; Training; Training and Education; Update; Vaccines; Viral; Viral Pathogenesis; base; chemical property; cost; instrument; instrumentation; member; novel strategies; tool

The overall objective of the Flow Cytometry Core is to efficiently provide CFAR members with comprehensive flow cytometry services, including equipment, expertise and training at a reasonable cost. Flow cytometry is a unique and powerful technology for determining the phenotype and function of cells and also allows defined populations of cells to be isolated by cell sorting. In recent years the use of flow cytometric analysis in HIV research has become even more prominent as new approaches for dissecting the interactions between HIV and cells of the immune system have been developed. The CFAR Flow Cytometry Core began operation in 1990 and continues to provide flow cytometry services to the AIDS researchers in the UAB community. During the last budget period 82 individual investigators used the flow cytometry core, including 28 CFAR members for AIDS-related projects. The instrumentation in the core has also recently been upgraded and now enables investigators to analyze six different parameters using three laser beams with possible emissions at 360 nm, 407 nm, 488nm, 528 nm, 568 nm, or 633nm. In addition the CFAR Flow Cytometry Core now also has available a FACScan instrument for five parameter, three color analysis. The specific aims of the flow cytometry core are: 1. Service. To provide AIDS Center members with cell sorting and analytical flow cytometry services, which are capable of handling potentially infectious materials. 2. Education and Training. To provide AIDS Center members with up to date information regarding the use of flow cytometry in AIDS research. 3. Development. A major goal of the flow cytometry core is to address users' needs by ensuring that AIDS Center members can take advantage of current technological advances and employ state of the art techniques in their research activities. Overall the CFAR Flow Cytometry Core provides key resources, expertise and support to AIDS investigators at UAB and acts as a focal point for numerous scientific interactions.

流式细胞术核心的总体目标是有效地为CFAR成员提供 全面的流式细胞仪服务，包括设备、专业知识和培训，费用合理。 流式细胞术是用于确定细胞的表型和功能的独特且强大的技术， 还允许通过细胞分选分离确定的细胞群。近年来，流量的使用 细胞计数分析在艾滋病毒研究中已经变得更加突出，因为新的方法用于解剖 HIV和免疫系统细胞之间的相互作用已经发展。CFAR流式细胞术 中心于1990年开始运作，并继续为艾滋病研究人员提供流式细胞术服务， UAB社区在上一个预算期间，82名调查员使用了流式细胞仪核心， 其中包括28名艾滋病相关项目的CFAR成员。堆芯的仪器最近也 现在，研究人员可以使用三束激光分析六种不同的参数 在360 nm、407 nm、488 nm、528 nm、568 nm或633 nm处具有可能的发射。此外，CFAR流程 Cytometry Core现在还提供FACScan仪器，用于五参数，三色分析。 流式细胞术核心的具体目标是： 1.的服务.为艾滋病中心会员提供细胞分选和流式细胞分析服务， 其能够处理潜在的传染性物质。 2.教育培训为艾滋病中心成员提供有关 流式细胞术在艾滋病研究中的应用。 3.发展流式细胞术核心的一个主要目标是通过确保艾滋病 中心成员可以利用当前的技术进步， 技术在他们的研究活动。 总的来说，CFAR流式细胞术核心为艾滋病提供了关键的资源、专业知识和支持 UAB的研究人员，并作为许多科学互动的焦点。