Flow Cytometry

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The overall objective of the Flow Cytometry Core is to efficiently provide CFAR members with comprehensive flow cytometry services, including equipment, expertise and training at a reasonable cost. Flow cytometry is a unique and powerful technology for determining the phenotype and function of cells and also allows defined populations of cells to be isolated by cell sorting. In recent years the use of flow cytometric analysis in HIV research has become even more prominent as new approaches for dissecting the interactions between HIV and cells of the immune system have been developed. The CFAR Flow Cytometry Core began operation in 1990 and continues to provide flow cytometry services to the AIDS researchers in the UAB community. During the last budget period 82 individual investigators used the flow cytometry core, including 28 CFAR members for AIDS-related projects. The instrumentation in the core has also recently been upgraded and now enables investigators to analyze six different parameters using three laser beams with possible emissions at 360 nm, 407 nm, 488nm, 528 nm, 568 nm, or 633nm. In addition the CFAR Flow Cytometry Core now also has available a FACScan instrument for five parameter, three color analysis. The specific aims of the flow cytometry core are: 1. Service. To provide AIDS Center members with cell sorting and analytical flow cytometry services, which are capable of handling potentially infectious materials. 2. Education and Training. To provide AIDS Center members with up to date information regarding the use of flow cytometry in AIDS research. 3. Development. A major goal of the flow cytometry core is to address users' needs by ensuring that AIDS Center members can take advantage of current technological advances and employ state of the art techniques in their research activities. Overall the CFAR Flow Cytometry Core provides key resources, expertise and support to AIDS investigators at UAB and acts as a focal point for numerous scientific interactions.