Flow Cytometry

亚拉巴马大学（DAB）艾滋病研究中心（CFAR）流式细胞术核心的主要目标是 提供最先进的流式细胞术服务，以支持艾滋病研究项目。流式细胞术是一种独特的， 强大的技术分析颗粒的荧光特性，并可以很容易地应用于确定 细胞的表型和功能，以及用于通过分选分离限定的细胞群体。近年来 在HIV研究中使用流式细胞仪分析变得越来越突出，因为新的解剖方法 已经开发了HIV和免疫系统细胞之间的相互作用;基于荧光的报告基因 已经利用系统来监测病毒复制、再活化和重组; 仪器的技术进步为高速多色分析提供了新的平台， 细胞分选因此，CFAR流式细胞术核心是促进和促进 艾滋病研究。流式细胞术核心的使命是通过提供 在一个组织良好的、集中的、能够处理潜在传染性的设施内使用必要的仪器 材料它提供培训机会和咨询，也是促进互动的渠道 研究者和实验室人员之间的利益和专业知识的不同。在过去的融资中 在此期间，CFAR流式细胞仪核心进行了重大的设备升级，安装了BDFACSAria 细胞分选仪以及BD-LSR-II分析流式细胞仪。流式细胞仪核心还改进了其 可见度和可获得性以及加强教育和培训举措。由于这些努力， 自2002年以来，流式细胞术核心的使用增加了三倍多。流式细胞仪核心具有 支持来自69个主要研究人员实验室的184名不同人员的研究活动， 在病毒发病机理、抗病毒研究、细胞免疫学、 免疫力和疫苗设计。核心基金支持了88项国家卫生研究院赠款的活动， 流式细胞术核心的目标是在我们迄今为止取得的进展的基础上， 服务、教育和培训活动，使用户能够充分利用先进的 可用的工具。这些努力包括根据需求评估分析， 第三流式细胞仪和生物荧光流式细胞仪，用于多路分析细胞中的生物分子（例如细胞因子）。 2008年初。我们还旨在通过与用户合作，促进合作和交叉评分来促进创新 鼓励与艾滋病有关的尖端研究活动的伙伴关系。

The major objective of the University of Alabama (DAB) Center for AIDS Research (CFAR) Flow Cytometry Core is to provide state-of-the-art flow cytometry services to support AIDS research programs. Flow cytometry is a unique and powerful technology for analyzing the fluorescent properties of particles and can be readily applied to determining the phenotype and function of cells, as well as utilized for isolating defined cell populations by sorting. In recent years the use of flow cytometric analyses in HIV research has become ever more prominent as newer approaches for dissecting the interactions between HIV and cells of the immune system have been developed; fluorescent based reporter systems have been harnessed to monitor virus replication, reactivation, and recombination; and sophisticated technological advances in instrumentation have provided new platforms for high-speed polychromatic analyses and cell sorting. The CFAR flow cytometry core is, therefore, a vital and versatile resource for promoting and facilitating AIDS research. The flow cytometry core's mission is to maximize the benefits of this technology by providing the necessary instrumentation within a well organized, centralized, facility that is capable of handling potentially infectious material. It provides training opportunities and consultations, and it also serves as a conduit for fostering interactions between investigators and laboratory personnel with diverse interests and expertise. During the previous funding period the CFAR flow cytometry core has undergone a significant equipment upgrade, with the installation of a BDFACSAria cell sorter as well as a BD-LSR-II analytical flow cytometer. The flow cytometry core has also improved its visibility and accessibility as well as enhanced education and training initiatives. As a result of these endeavors the usage of the flow cytometry core has increased more than three-fold since 2002. The flow cytometry core has supported the research activities of 184 different individuals from 69 Principle Investigators laboratories and has contributed to basic, clinical, and translational initiatives in the areas of viral pathogenesis, anti-viral research, cellular immunity, and vaccine design. The core has supported the activities of 88 NIH grants and contributed to 172 publications The aims of the flow cytometry core are to now build upon our progress-to-date by further enhancing services, and education and training activities so that users are empowered to take full advantage of the sophisticated instrumentation that is available. These efforts include, based on a needs assessment analysis, the installation of a third flow cytometer and a BioPlex flow cytometer for multiplexed analysis of biomolecules (e.g. cytokines) in the beginning of 2008. We also aim to promote innovation by working with users, and fostering collaborations and crosscore partnerships that encourage cutting-edge AIDS related research activities.