The role of creatine in health and disease

肌酸在健康和疾病中的作用

• 批准号: 7348098
• 负责人: WILLIAM James CRAIGEN
• 金额: $ 20.03万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7348098
• 关键词: Address; Affect; Amyotrophic Lateral Sclerosis; Anabolism; Animals; Area; Biochemical; Bioenergetics; Biological Assay; Brain; Cell Death; Clinical; Creatine; Creatine Kinase; Deformity; Disease; Disease model; Early treatment; Electrophysiology (science); Enzymes; Exercise Tolerance; Exercise stress test; Fiber; Functional disorder; Glycine amidinotransferase; Goals; Growth; Health; Hereditary Disease; Homeostasis; Human; Huntington Disease; Hypoxia; Inborn Errors of Metabolism; Indirect Calorimetry; Infant; Inherited; Injury; Ischemic Brain Injury; Isoenzymes; Learning; Liquid substance; Magnetic Resonance Spectroscopy; Mental Retardation; Metabolic Diseases; Mitochondria; Modeling; Mus; Muscle; Muscle Fibers; Neonatal Screening; Neurologic; Neurons; Neuroprotective Agents; Newborn Infant; Oral; Organ; Parkinson Disease; Pathologic; Pathway interactions; Patients; Pharmaceutical Preparations; Phenotype; Phosphocreatine; Physiological; Polarography; Predisposition; Preparation; Principal Investigator; Production; Research; Research Personnel; Respiration; Respiratory Chain; Role; Seizures; Skin; Slice; Stress; Supplementation; Synaptic plasticity; Tissues; behavior test; classical conditioning; conditioned fear; insight; interest; morris water maze; mouse model; mutant; nervous system disorder; novel; prevent; prophylactic; protective effect; response

DESCRIPTION (provided by applicant): Creatine (Cr) is an essential component of cellular energy storage and production in tissues with high energy requirements. Creatine deficiency in humans is caused by a small group of rare inborn errors of metabolism involving synthesis and transport. In contrast, creatine supplementation is purported to have a protective effect on neurologic progression in disease models of amyotrophic lateral sclerosis, Huntington's disease, and Parkinson's disease. Furthermore, prophylactic creatine administration in animals appears to be neuroprotective in models of traumatic and ischemic brain injuries. The most significant utilization of creatine occurs in muscle and brain. In these organs, creatine and phosphocreatine, together with creatine kinase isozymes and ATP/ADP, provide the major reserve of energy. Deficiency of the first enzyme in the creatine synthetic pathway, arginine:glycine amidinotransferase (AGAT), in humans leads to complete deficiency of creatine synthesis and severe neurological sequelae, including mental retardation and autistic features. However, newborns appear normal and early treatment may eliminate neurologic injury. Thus, creatine deficiency appears to be an excellent candidate for newborn screening. Using a mouse model of AGAT deficiency, the goal of this application is to provide insights into the pathologic mechanisms of this inherited metabolic disease with regard to learning and synaptic plasticity. The investigators will use behavioral testing in conjunction with electrophysiology to examine the neurologic phenotype and its response to creatine supplementation. They are also interested in understanding the effect of creatine deficiency on muscle bioenergetics using various physiologic and biochemical assays, including exercise testing, tissue and fluid analytes, magnetic resonance spectroscopy, and mitochondrial respiration with skinned muscle fibers. Also to be investigated are the effects of oral creatine administration as a neuroprotective agent by determining the threshold for seizure induction and sensitivity to hypoxic injury. These studies will provide insights into a screenable human disorder as well as broader biomedical issues.

描述（由申请人提供）：肌酸（Cr）是高能量需求组织中细胞能量储存和生产的重要组成部分。人类肌酸缺乏是由一小部分罕见的先天性代谢错误引起的，包括合成和运输。相反，在肌萎缩侧索硬化症、亨廷顿氏病和帕金森病的疾病模型中，补充肌酸被认为对神经系统进展有保护作用。此外，动物预防性肌酸管理似乎对创伤性和缺血性脑损伤模型具有神经保护作用。