Designer Retinal Circuits: Interfacing optical tweezers with an electronic device

描述（由申请人提供）：我们的目标是检查视网膜回路的发育和重建：1）如果要恢复某些视力，则必须在视网膜移植后形成，2）当视网膜重塑时发生在退化视网膜中，以及3）在正常视网膜中起作用。我们建议这样做，结合技术的光镊与微电极阵列上的玻璃芯片。芯片将被设计为具有分别用于细胞移动和细胞培养的非粘附区和粘附区。使用光学镊子，通过其独特的形态识别的分离的成年视网膜细胞将被显微操纵到电极上，并允许形成细胞与细胞的接触。将通过免疫细胞化学和突触囊泡再循环染料监测突触发育。将通过电极刺激和双极细胞和一些无长突细胞的无毒电压敏感染料成像，以及通过记录无长突细胞和神经节细胞的尖峰和动作电位的电极来测试突触活性。镊子的使用允许对构建的电路进行精确控制。随着时间的推移，设计和功能测试特定神经细胞相互作用的能力将为系统提供前所未有的实验控制水平。

DESCRIPTION (provided by applicant): Our goals are to examine the development and reconstruction of retinal circuits 1) that must form after retinal transplantation if some vision is to be restored, 2) that occur in the degenerate retina as the retina is remodeled, and 3) that function in the normal retina. We propose to do this by combining the technologies of optical tweezers with microelectrode arrays on glass chips. Chips will be designed to have non-adherent and adherent zones for cell movement and cell culture respectively. Using the optical tweezers, isolated adult retinal cells that are identified by their distinctive morphologies will be micromanipulated onto electrodes and allowed to form cell-cell contacts. Synaptic development will be monitored by immunocytochemistry and synaptic vesicle recycling dyes. Synaptic activity will be tested by electrode stimulation and imaging of non- toxic voltage-sensitive dyes for bipolar and some amacrine cells, and by recording electrodes for the spiking and action potentials of amacrine and ganglion cells. The use of the tweezers allows precise control over the circuits that are built. The ability to design and then functionally test specific nerve cell interactions over time will provide a system with an unprecedented level of experimental control.