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CTRP Transgenics and Knockouts as Models of Macular Degeneration

CTRP 转基因和基因敲除作为黄斑变性模型

基本信息

批准号：
7494006
负责人：
Robert M Petters
金额：
$ 21.59万
依托单位：
NORTH CAROLINA STATE UNIVERSITY RALEIGH
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-07 至 2010-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7494006
关键词：
Affect Age Age related macular degeneration Alleles Anatomy Animal Model Animals Blindness Breeding Cardiovascular Diseases Cells Ciliary epithelium Cloning Condition Dark Adaptation Deposition Development Diet Dominant-Negative Mutation Elderly Evaluation Exposure to Eye Family suidae Gender Gene Expression Gene Mutation Generations Genes Genotype Goals Growth and Development function Histology Human Immunohistochemistry Incidence Individual Knock-out Macular degeneration Membrane Methods Modeling Morphology Mus Mutation Phenotype Physiology Process Production Protein Overexpression Race Report (document)Retinal Retinal Degeneration Staging Structure of retinal pigment epithelium Sunlight Sus scrofa Testing Therapeutic Tissues Transgenic Organisms cigarette smoking human disease man molecular pathology neovascular novel promoter size somatic cell nuclear transfer therapy development

项目摘要

DESCRIPTION (provided by applicant): The long-term objective of this project is to understand the cause(s) and mechanisms of retinal degeneration in age-related macular degeneration. The most common form of blindness in older adults, macular degeneration treatments are relatively limited because little is known about the degenerative process. The pig is a superior animal model because the size and retinal anatomy and physiology are similar to that in humans. The specific aim of this project is to produce and determine the extent to which CTRP5 transgenic and knockout pigs will serve as a valid model for macular degeneration in humans. Transgenic and knockout pigs will be produced using somatic cell nuclear transfer. Eyes from transgenic and knockout pigs of various ages will be studied. Histological analysis will document the type and extent of retinal degeneration. Results obtained with the transgenic and knockout pigs will be compared with known phenotypes in man and other model species such as the mouse. Animal models are very important for developing any treatments to slow the progress of macular degeneration. Transgenic pigs will provide more information about macular degeneration and will allow for testing therapies to slow the progress of macular degeneration.

描述（由申请人提供）：本项目的长期目标是了解年龄相关性黄斑变性中视网膜变性的原因和机制。老年人失明的最常见形式，黄斑变性治疗相对有限，因为对退行性过程知之甚少。猪是一种上级动物模型，因为其大小和视网膜解剖学和生理学与人类相似。该项目的具体目的是生产和确定CTRP 5转基因和敲除猪将在多大程度上作为人类黄斑变性的有效模型。转基因和基因敲除猪将使用体细胞核移植生产。将研究来自不同年龄的转基因和基因敲除猪的眼睛。组织学分析将记录视网膜变性的类型和程度。用转基因和基因敲除猪获得的结果将与人和其他模型物种（如小鼠）中的已知表型进行比较。动物模型对于开发任何减缓黄斑变性进展的治疗方法都非常重要。转基因猪将提供更多关于黄斑变性的信息，并将允许测试治疗以减缓黄斑变性的进展。