ANIMAL BREEDING

动物饲养

• 批准号: 7459709
• 负责人: Laura Haynes
• 金额: $ 14.47万
• 依托单位: TRUDEAU INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7459709
• 关键词: Age; Aging; Animals; Backcrossings; Cell physiology; Core Facility; Ensure; Generations; Genetic; Health; Human Resources; Immune system; Inbred Strain; Institutes; Kinetics; Knock-out; Longevity; Modeling; Mus; Numbers; Population; Research Personnel; Screening procedure; Services; T-Lymphocyte; Time; Transgenic Mice; Transgenic Organisms; age effect; aged; animal breeding; animal facility; biosafety level 3 facility; healthy aging; knockout animal; mouse model; programs; pup; research study

Mice provide a well-studied homogeneous population that can be manipulated readily for experiments examining the effect of age on the immune system. By using murine models to study aging, we can easily examine different components of the immune system. Furthermore, the availability of genetically manipulated mouse models will allow us to study the effect of age on a single parameter at a time. This Core Facility will provide services not currently provided by the Trudeau Institute animal facility. The specific aims of Core B will be: (1) generation of a colony of healthy aged mice - the Core will ensure that aged mice are maintained on ventilated racks and that monthly health screenings are performed; (2) generation of new inbred strains of genetically altered mice - the Core will backcross knockout and transgenic mice onto appropriate genetic backgrounds, as needed by each investigator. This Core will also ensure that all mice needed by the investigators in the Program are screened in a timely manner. These mice can then be purchased by each investigator.

小鼠提供了一个良好的均匀人群，可以轻松地操纵研究年龄对免疫系统的影响的实验。通过使用鼠模型来研究衰老，我们可以轻松检查免疫系统的不同组成部分。此外，遗传操纵小鼠模型的可用性将使我们一次研究年龄对单个参数的影响。该核心设施将提供特鲁多研究所动物设施目前未提供的服务。核心B的具体目的是：（1）生成健康老化小鼠的菌落 - 核心将确保将老化的小鼠保持在通风架上，并进行每月的健康检查； （2）新的近交菌株的产生 遗传改变的小鼠 - 核心将根据每个研究者的需要，将折叠敲除和转基因小鼠回到适当的遗传背景上。该核心还将确保及时筛选程序中调查人员所需的所有小鼠。然后可以由每个研究者购买这些老鼠。