ANIMAL CORE

动物核心的目标是提供良好的特征动物，以维持，饲养，监测， 分析本计划项目科学项目所需的动物，并建立新的 转基因株系，用于今后深入研究朊病毒疾病。这些目标将按照 以下四个目标，通过设计，产生/获取，表征，维护，育种， 所有动物的接种、监测和初步分析（接种后）。 动物核心的功能将通过四个具体目标来实现。 目标1：为所有动物提供专业的饲养和可靠和一致的护理。至关重要 由于需要大量的动物和广泛的杂交育种， 并且ABSL 3设施中的动物在接种后通常需要长时间保持。 目的2：设计、生成/获取、表征和维持独特的转基因小鼠品系，以满足 整个项目的需求。为本项目和未来的深入研究开发新的转基因小鼠品系 朊病毒疾病的研究。许多转基因小鼠已经开发出来，有些将被创建或 后天的所有转基因小鼠将在释放给研究者之前进行充分表征。 目标3：用各种接种物接种动物（小鼠或仓鼠），监测和记录健康状况， 症状，并解剖动物以获得用于分析的组织/流体。将创建一个数据库， 保存朊病毒ABSL 3设施中每只动物的所有信息。 目的4：对接种动物的组织/体液进行蛋白质印迹分析，以评估 感染，并为研究者提供表征的组织;与组织核心密切合作， 在用朊病毒材料接种之前和之后表征所有动物。 在这个核心中生产的许多转基因动物都是独一无二的，将成为整个地球的宝贵资源。 朊病毒研究和监控社区。

The goals of the Animal Core are to provide well characterizedanimals, to maintain, inoculate, monitor, and analyze the animals required for the scientific projects of this Program Project,and to establish new transgenic lines for future in-depth study of prion diseases. These goals will be accomplished as specifiedin the following four Aims through design, generation/acquisition, characterization, maintenance, breeding, inoculation, monitoring, and preliminary analysis (after inoculation) of all the animals. The function of the Animal Core will be achieved through four specific aims. Aim 1: Provide professional husbandry and reliable and consistent care to all the animals. It is critical that the animals receive reliable care since a large number of animals and extensive cross-breeding are required and animals in the ABSL3 facility often need to be maintained for long periods of time after inoculation. Aim 2: Design, generate/acquire, characterize, and maintain unique transgenic mouse lines to meet the needs of the entire Program Project. Develop new transgenic mouse lines for this project and future in-depth study of prion diseases. Many transgenic mice have been developed previously, and some will be created or acquired. All transgenic mice will be fully characterized before release to the investigators. Aim 3: Inoculate animals (mice or hamsters) with a variety of inoculum, monitor and record health status and symptoms, and dissect animals to obtain tissues/fluids for analysis. A database will be created and maintained to store all the information for each animal in the prion ABSL3 facility. Aim 4: Conduct Western blot analysis of tissues/fluids from the inoculated animals to assess the status of infection and to provide characterized tissues for the investigators; work closely with the Tissue Core to fully characterize all the animals before and after inoculation with prion materials. Many of the transgenic animals produced in this Core are unique and will be valuable resources to the entire prion research and surveillance community.