ANIMAL CORE

动物核心的目标是提供具有良好特征的动物，以维持、接种、监测和 分析本项目科研项目所需的动物，并建立新的 转基因品系为今后深入研究病毒病奠定了基础。这些目标将按照指定的方式实现 通过设计、生成/获取、表征、维护、育种、 对所有动物进行接种、监测和初步分析(接种后)。 动物核心的功能将通过四个具体目标实现。 目标1：为所有动物提供专业的饲养和可靠、一致的护理。至关重要的是 这些动物得到了可靠的照顾，因为需要大量的动物和广泛的杂交 而ABSL3设施中的动物在接种后往往需要长时间饲养。 目标2：设计、生成/获取、鉴定和维护独特的转基因小鼠品系，以满足 整个计划项目的需求。为该项目和未来的深入研究开发新的转基因小鼠品系 Pron疾病的研究。之前已经开发了许多转基因小鼠，其中一些将被创造出来或 获得者。所有转基因小鼠在被释放给研究人员之前都将得到充分的鉴定。 目标3：给动物(小鼠或仓鼠)接种各种疫苗，监测和记录健康状况和 症状，并解剖动物以获取组织/体液进行分析。将创建一个数据库，并 进行维护，以便在Prion ABSL3设施中存储每种动物的所有信息。 目的4：对接种动物的组织/体液进行蛋白质印迹分析，以评估其状态。 感染并为调查人员提供特征性组织；与组织核心密切合作，以充分 对所有动物接种普恩蛋白前后的特征进行分析。 在这个核心生产的许多转基因动物都是独一无二的，将成为整个 普里恩研究和监控界。

The goals of the Animal Core are to provide well characterizedanimals, to maintain, inoculate, monitor, and analyze the animals required for the scientific projects of this Program Project,and to establish new transgenic lines for future in-depth study of prion diseases. These goals will be accomplished as specifiedin the following four Aims through design, generation/acquisition, characterization, maintenance, breeding, inoculation, monitoring, and preliminary analysis (after inoculation) of all the animals. The function of the Animal Core will be achieved through four specific aims. Aim 1: Provide professional husbandry and reliable and consistent care to all the animals. It is critical that the animals receive reliable care since a large number of animals and extensive cross-breeding are required and animals in the ABSL3 facility often need to be maintained for long periods of time after inoculation. Aim 2: Design, generate/acquire, characterize, and maintain unique transgenic mouse lines to meet the needs of the entire Program Project. Develop new transgenic mouse lines for this project and future in-depth study of prion diseases. Many transgenic mice have been developed previously, and some will be created or acquired. All transgenic mice will be fully characterized before release to the investigators. Aim 3: Inoculate animals (mice or hamsters) with a variety of inoculum, monitor and record health status and symptoms, and dissect animals to obtain tissues/fluids for analysis. A database will be created and maintained to store all the information for each animal in the prion ABSL3 facility. Aim 4: Conduct Western blot analysis of tissues/fluids from the inoculated animals to assess the status of infection and to provide characterized tissues for the investigators; work closely with the Tissue Core to fully characterize all the animals before and after inoculation with prion materials. Many of the transgenic animals produced in this Core are unique and will be valuable resources to the entire prion research and surveillance community.