Vortex Tubed Thermocycler with Intelligent Software

带智能软件的涡流管热循环仪

• 批准号: 7460829
• 负责人: HENDRIK J VILJOEN
• 金额: $ 36.32万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7460829
• 关键词: Applications Grants; Automobile Driving; Bacillus anthracis; Bacillus cereus; Base Pairing; Biochemistry; Chemical Engineering; Communicable Diseases; Computer software; Condition; Cytomegalovirus; DNA; DNA amplification; DPYD gene; Detection; Devices; Diagnosis; Dihydropyrimidine Dehydrogenase; Electronics; Engineering; Forensic Medicine; Gases; Genes; Heating; Hematology; Human; Liquid substance; Mechanics; Modeling; Nebraska; Optics; Organ Transplantation; Organic Chemistry; Oxidoreductase; Performance; Pharmacogenetics; Phase; Polymerase; Polymerase Chain Reaction; Process; Reaction; Research; Speed; System; Temperature; Testing; Time; Transplantation; Tube; Universities; Viral; base; concept; cost; improved; insight; instrument; mathematical model; novel; oncology; point of care; portability; tool; virtual

DESCRIPTION (provided by applicant): A novel system is proposed for the rapid identification of DNA. The system comprises of a unique thermocycler platform built around the extraordinary vortex tube, detection optics, intelligent software to provide users with information on most ideal operating conditions, and virtual insight into the PCR process as it progresses. An intelligent user interface will allow DNA amplification/detection on an unprecedented timescale (less than 10 minutes). A multi-disciplinary team that consists of a chemical engineer, a mechanical engineer, and two biochemists has been assembled for this project. The efficiency of the vortex tube will be optimized by the use of computational fluid dynamics. Heat transfer between the gas phase and the cuvets will be improved through computational fluid dynamic calculations. The intelligent software consists of a detailed mathematical model that uses similar starting conditions as the initial cuvet composition to model the amplification progress and it performs a virtual PCR in parallel with the actual process. The virtual PCR will become a quantitative tool point-of-care diagnosis of a wide variety of heritable and infectious diseases. The virtues of the intelligent vortex tube PCRJet are: speed, versatility, reliability, portability and low cost.

描述（由申请人提供）：提出了一种用于快速鉴定DNA的新系统。该系统包括一个独特的热循环仪平台，围绕非凡的涡流管，检测光学，智能软件，为用户提供最理想的操作条件的信息，并虚拟洞察PCR过程中的进展。智能用户界面将允许在前所未有的时间尺度上（不到10分钟）进行DNA扩增/检测。一个由化学工程师，机械工程师和两名生物化学家组成的多学科团队已经为这个项目组装。涡流管的效率将通过使用计算流体动力学来优化。通过计算流体动力学计算，将改善气相和比色皿之间的传热。智能软件由一个详细的数学模型组成，该模型使用与初始比色皿组成相似的起始条件来模拟扩增过程，并与实际过程并行执行虚拟PCR。虚拟PCR将成为各种遗传性和传染性疾病的定量即时诊断工具。智能涡流管PCRJet的优点是：速度快，通用性强，可靠性高，便携性好，成本低。

项目成果

A Kinetic Study of In Vitro Lysis of Mycobacterium smegmatis.

• DOI: 10.1016/j.ces.2008.12.015
• 发表时间: 2009-05-01
• 期刊: Chemical engineering science
• 影响因子: 4.7
• 作者: Valente W;Pienaar E;Fast A;Fluitt A;Whitney S;Fenton R;Barletta R;Chacon O;Viljoen H
• 通讯作者: Viljoen H

A model of tuberculosis transmission and intervention strategies in an urban residential area.

• DOI: 10.1016/j.compbiolchem.2010.03.003
• 发表时间: 2010-04
• 期刊: COMPUTATIONAL BIOLOGY AND CHEMISTRY
• 影响因子: 3.1
• 作者: Pienaar, Elsje;Fluitt, Aaron M.;Whitney, Scott E.;Freifeld, Alison G.;Viljoen, Hendrik J.
• 通讯作者: Viljoen, Hendrik J.