Vortex Tubed Thermocycler with Intelligent Software

带智能软件的涡流管热循环仪

• 批准号: 7250169
• 负责人: HENDRIK J VILJOEN
• 金额: $ 35.51万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7250169
• 关键词: Chemical Engineering; Communicable Diseases; Computer software; Condition; DNA; DNA amplification; Detection; Diagnosis; Engineering; Gases; Heating; Liquid substance; Mechanics; Modeling; Optics; Phase; Polymerase Chain Reaction; Process; Speed; System; Tube; cost; improved; insight; mathematical model; novel; point of care; portability; tool; virtual

DESCRIPTION (provided by applicant): A novel system is proposed for the rapid identification of DNA. The system comprises of a unique thermocycler platform built around the extraordinary vortex tube, detection optics, intelligent software to provide users with information on most ideal operating conditions, and virtual insight into the PCR process as it progresses. An intelligent user interface will allow DNA amplification/detection on an unprecedented timescale (less than 10 minutes). A multi-disciplinary team that consists of a chemical engineer, a mechanical engineer, and two biochemists has been assembled for this project. The efficiency of the vortex tube will be optimized by the use of computational fluid dynamics. Heat transfer between the gas phase and the cuvets will be improved through computational fluid dynamic calculations. The intelligent software consists of a detailed mathematical model that uses similar starting conditions as the initial cuvet composition to model the amplification progress and it performs a virtual PCR in parallel with the actual process. The virtual PCR will become a quantitative tool point-of-care diagnosis of a wide variety of heritable and infectious diseases. The virtues of the intelligent vortex tube PCRJet are: speed, versatility, reliability, portability and low cost.

描述（由申请人提供）：提出了一种用于快速鉴定DNA的新颖系统。该系统由围绕非凡涡流管构建的独特热循环仪平台、检测光学器件、智能软件组成，可为用户提供有关最理想操作条件的信息，以及对 PCR 过程进展的虚拟洞察。智能用户界面将允许在前所未有的时间尺度（不到 10 分钟）内进行 DNA 扩增/检测。该项目组建了一个由一名化学工程师、一名机械工程师和两名生物化学家组成的多学科团队。涡流管的效率将通过计算流体动力学的使用来优化。通过计算流体动力学计算将改善气相和比色皿之间的热传递。智能软件由详细的数学模型组成，该模型使用与初始比色杯成分类似的起始条件来模拟扩增进度，并与实际过程并行执行虚拟 PCR。虚拟PCR将成为多种遗传性疾病和传染病的即时诊断定量工具。智能涡流管PCRJet的优点是：速度快、多功能性、可靠性、便携性和低成本。