Vortex Tubed Thermocycler with Intelligent Software

描述(申请人提供)：提出了一种用于DNA快速鉴定的新系统。该系统包括一个围绕特殊涡流管构建的独特的热循环仪平台、检测光学装置、为用户提供最理想操作条件信息的智能软件，以及对聚合酶链式反应过程的虚拟洞察。智能用户界面将允许在前所未有的时间范围内(不到10分钟)进行DNA扩增/检测。一个由一名化学工程师、一名机械工程师和两名生物化学家组成的多学科团队已经为这个项目组建起来。涡流管的效率将通过使用计算流体力学进行优化。通过计算流体力学计算，可以改善气相与切割器之间的换热。智能软件由一个详细的数学模型组成，该模型使用类似的起始条件作为初始CUVE成分来模拟扩增过程，并与实际过程并行执行虚拟PCR。虚拟聚合酶链式反应将成为一种定量工具，用于诊断各种遗传性和传染性疾病。智能涡流管PCRJet的优点是：速度快、通用性强、可靠性高、便携性好、成本低。

DESCRIPTION (provided by applicant): A novel system is proposed for the rapid identification of DNA. The system comprises of a unique thermocycler platform built around the extraordinary vortex tube, detection optics, intelligent software to provide users with information on most ideal operating conditions, and virtual insight into the PCR process as it progresses. An intelligent user interface will allow DNA amplification/detection on an unprecedented timescale (less than 10 minutes). A multi-disciplinary team that consists of a chemical engineer, a mechanical engineer, and two biochemists has been assembled for this project. The efficiency of the vortex tube will be optimized by the use of computational fluid dynamics. Heat transfer between the gas phase and the cuvets will be improved through computational fluid dynamic calculations. The intelligent software consists of a detailed mathematical model that uses similar starting conditions as the initial cuvet composition to model the amplification progress and it performs a virtual PCR in parallel with the actual process. The virtual PCR will become a quantitative tool point-of-care diagnosis of a wide variety of heritable and infectious diseases. The virtues of the intelligent vortex tube PCRJet are: speed, versatility, reliability, portability and low cost.