Vortex Tubed Thermocycler with Intelligent Software

带智能软件的涡流管热循环仪

• 批准号: 6914863
• 负责人: HENDRIK J VILJOEN
• 金额: $ 16.79万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6914863
• 关键词: DNA; artificial intelligence; bacterial DNA; bioengineering /biomedical engineering; bioinformatics; biomedical equipment development; computational biology; computer program /software; computer system design /evaluation; diagnosis design /evaluation; diagnostic tests; mathematical model; neoplasm /cancer genetics; nucleic acid amplification techniques; nucleic acid purification; nucleic acid quantitation /detection; optics; polymerase chain reaction; portable biomedical equipment; virus DNA

DESCRIPTION (provided by applicant): A novel system is proposed for the rapid identification of DNA. The system comprises of a unique thermocycler platform built around the extraordinary vortex tube, detection optics, intelligent software to provide users with information on most ideal operating conditions, and virtual insight into the PCR process as it progresses. An intelligent user interface will allow DNA amplification/detection on an unprecedented timescale (less than 10 minutes). A multi-disciplinary team that consists of a chemical engineer, a mechanical engineer, and two biochemists has been assembled for this project. The efficiency of the vortex tube will be optimized by the use of computational fluid dynamics. Heat transfer between the gas phase and the cuvets will be improved through computational fluid dynamic calculations. The intelligent software consists of a detailed mathematical model that uses similar starting conditions as the initial cuvet composition to model the amplification progress and it performs a virtual PCR in parallel with the actual process. The virtual PCR will become a quantitative tool point-of-care diagnosis of a wide variety of heritable and infectious diseases. The virtues of the intelligent vortex tube PCRJet are: speed, versatility, reliability, portability and low cost.

描述（由申请人提供）：提出了一种快速鉴定DNA的新系统。该系统包括一个独特的热循环器平台，围绕非凡的涡流管，检测光学，智能软件，为用户提供最理想的操作条件的信息，以及虚拟的洞察PCR过程的进展。智能用户界面将允许DNA扩增/检测在前所未有的时间尺度（不到10分钟）。这个项目已经组建了一个由一名化学工程师、一名机械工程师和两名生物化学家组成的多学科团队。利用计算流体力学的方法对涡流管的效率进行了优化。通过计算流体动力学计算，将改善气相和隔板之间的传热。智能软件由一个详细的数学模型组成，该模型使用与初始培养皿组成相似的起始条件来模拟扩增过程，并与实际过程并行执行虚拟PCR。虚拟PCR将成为多种遗传性和传染性疾病即时诊断的定量工具。智能涡旋管PCRJet的优点是：速度快、通用性强、可靠性高、便携性好、成本低。