CORE--MASS SPECTOMETRY

核心——质谱

• 批准号: 7470674
• 负责人: UWE CHRISTIANS
• 金额: $ 18.71万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7470674
• 关键词: Animals; Arts; Biochemical; Biological Assay; Cells; Complex; Computer software; Diagnostic; Disease; Energy Metabolism; Equipment; Gases; High Pressure Liquid Chromatography; Instruction; Isotopes; Magnetic Resonance Imaging; Mass Spectrum Analysis; Measures; Metabolism; Methodology; Minerals; Mission; Modeling; Monitor; Nutrient; Nutritional Study; Proteomics; Purpose; Range; Research; Research Personnel; Services; Technology; Time; Tissues; Two-Dimensional Gel Electrophoresis; absorption; instrument; instrumentation; interest; laboratory facility; metabolomics; stable isotope; success; tool

The mission of the core is to provide analytical services and to develop new assays and technology to support CNRU investigators to develop an understanding of nutrient absorption, metabolism, and turnover. The Mass Spectrometry Core furnishes instruments and technologies that would not normally be available to most independent investigators and is, in fact, essential to the success of these investigators. The core is also developing diagnostic tools to predict disease and to monitor progress of disease and treatment. In addition, we provide comprehensive instruction for interested CNRU investigators in order to increase their proficiency in our highly complex technologies and instrumentation. The range of instrumentation available to CNRU investigators through the Core has significantly expanded during the last year and now includes GC-MS, isotope ratio MS, LC-MS, LC-MS/MS, LCMS/iontrap (with all components required for proteomics research), time-of-flight mass spectrometry, two-dimensional gel electrophoresis equipment and software, preparative HPLC with MS-guided automatic autosampler, 2 NMR spectrometers for biochemical profiling of cell and tissue metabolism (metabolomics) and animal MRI.

核心的使命是提供分析服务，并开发新的测定和技术，以支持CNRU研究人员了解营养吸收，代谢和周转。质谱核心涵盖了大多数独立调查人员通常无法获得的仪器和技术，事实上，这对这些调查人员的成功至关重要。该中心还在开发诊断工具，以预测疾病并监测疾病和治疗的进展。此外，我们为感兴趣的CNRU调查人员提供全面的指导，以提高他们对我们高度复杂的技术和仪器的熟练程度。CNRU研究人员通过核心获得的仪器范围在过去一年中显著扩大，现在包括GC-MS，同位素比MS，LC-MS，LC-MS/MS，LCMS/离子阱（具有蛋白质组学研究所需的所有组件）、飞行时间质谱、二维凝胶电泳设备和软件、具有MS引导的自动自动进样器的制备型HPLC、2台NMR光谱仪，用于细胞和组织代谢的生化分析（代谢组学）和动物MRI。