IMAGING, DATA ANALYSIS AND ACUISITION MODULE

概述 成像、数据分析和采集模块的总体用途(称为 模块‘)是双重的。首先，它将为最先进的静态和动态成像提供设施和支持 来自人类和非人类视觉系统不同区域的固定和活组织。其次，它将 促进开发先进和高度专业化的数据采集和分析软件。与 完成成像到数字方法的转换，先进的数据采集和分析技术 需要定量检查视觉系统的结构、发育和功能。此模块提供 图像采集的通用硬件，以及数据采集和数据采集的软件开发和支持 分析。 在过去的十年中，数字成像技术及其在Basic中的应用发生了革命 生物医学研究。成像模块一直是将这项技术带入视野的关键资源 耶鲁大学的社区。高级成像设备价格昂贵，通常无法购买 仅靠一名调查员的资源进行维护。愿景核心计划的存在以及这一点 耶鲁大学的成像模块一直是显微镜和相关产品开发和采购的杠杆 设备使用耶鲁大学的资源，并辅之以愿景计划资金。这使得耶鲁大学 视觉研究人员采用新的工具和方法来发现视觉科学。在上一个授权期内，我们的 成像模块在几个方面进行了扩展。首先，我们的奥林巴斯倒置共焦显微镜显著地 升级了。其次，我们为我们的标准立柱购买了SPOT数码相机和相关的SPOT软件 (蔡司Axiovert)和倒置(Zeiss Axioskop)显微镜。这些改进简化了图像 在不影响质量的情况下为视觉研究人员提供采集和分析，从而显著拓宽 耶鲁大学视觉科学研究人员对这些核心设施的用户基础。

Overview The overall purpose of the Imaging, Data Analysis and Acquisition Module (referred to as the 'Imaging Module') is two-fold. First, it will provide facilities and support for state-of-the art static and dynamic imaging of fixed and living tissue from a variety of regions of the human and non-human visual system. Second, it will facilitate the development of advanced and highly specialized data acquisition and analysis software. With the complete conversion of imaging to digital methods, sophisticated data acquisition and analysis techniques are required to quantitatively examine visual system structure, development and function. This module supplies common hardware for image acquisition, as well as software development and support for data acquisition and analysis. Over the past decade there has been a revolution in digital imaging technology and its application to basic biomedical research. The Imaging Module has been a critical resource in bringing this technology to the vision community at Yale. Advanced imaging equipment is expensive and typically cannot be purchased and maintained with the resources of a single investigator. The existence of the Vision Core Program and this Imaging Module at Yale has served as leverage for the development and acquisition of microscopes and related equipment using Yale University resources supplemented by Vision Program funds. This has enabled Yale vision researchers to adopt new tools and approaches to vision science discoveries. In the last grant period, our Imaging Module expanded in several ways. First, our Olympus inverted confocal microscope was significantly upgraded. Second, we acquired SPOT digital cameras and associated SPOT software for our standard upright (Zeiss Axiovert) and inverted (Zeiss Axioskop) microscopes. These improvements have simplified image acquisition and analysis for vision researchers without compromising quality, thereby significantly broadening the user base among vision science researchers at Yale for these core facilities.