Facility Core E: Cell & Tissue Analysis

设施核心 E：单元

• 批准号: 7239197
• 负责人: Ellen R Richie
• 金额: $ 7.66万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7239197
• 关键词: Apoptotic; Cell Cycle; Cell Separation; Cells; Complement; Confocal Microscopy; Consultations; Core Facility; Cytoplasmic Protein; Flow Cytometry; Localized; Organelles; Research Personnel; Resolution; Services; Subcellular structure; Surface; Suspension substance; Suspensions; Tissues; Training; base; cell type; digital imaging; intracellular protein transport; protein expression; protein localization location; research study; single cell analysis

A. Overall Objectives And Specific Aims The Cell and Tissue Analysis Facility Core includes flow cytometry and confocal microscopy services. The flow cytometry service provides Center investigators with the ability to quantify surface and/or cytoplasmic protein expression in a variety of cell types, analyze the fraction of apoptotic cells, evaluate cell cycle parameters and sort cells into purified subsets based on these and other criteria. Confocal microscopy complements the quantitative advantages of flow cytometry by localizing cells, organelles or other subcellular components that express particular surface or cytoplasmic proteins in tissue sections using high-resolution digital imaging. The Specific Aims of the Cell and Tissue Analysis Facility Core are: 1. To utilize flow cytometry for multiparameter analysis of single cell suspensions and for isolation of purified subsets by cell sorting 2. To utilize confocal microscopy for precise localization of proteins and subcellular structures. 3. To provide training and consultation on planning, executing and evaluating experiments involving flow cytometry and confocal microscopy

A.总体目标和具体目标