TISSUE CULTURE AND HYBRIDOMA MODULE

组织培养和杂交瘤模块

• 批准号: 7286544
• 负责人: Ram H Nagaraj
• 金额: $ 12.63万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7286544
• 关键词: American Type Culture Collection; Antigens; Area; Blood capillaries; Bos taurus; Capillary Endothelial Cell; Cattle; Cell Line; Cell Maintenance; Cells; Condition; Cornea; Cryopreservation; Cultured Cells; Development; Disease; Endothelial Cells; Ensure; Epithelial Cells; Equipment; Eye Banks; Faculty; Funding; Generations; Histology; Human; Hybridomas; Image; Incubators; Maintenance; Microscopy; Monoclonal Antibodies; Mus; Mycoplasma; Oryctolagus cuniculus; Pericytes; Primary Cell Cultures; Proteins; Purpose; Rattus; Recruitment Activity; Research Personnel; Retinal; Retinal Ganglion Cells; Running; Services; Source; Structure of retinal pigment epithelium; Testing; Tissues; Training; Transfection; Umbilical vein; University Hospitals; Work; capillary; cell type; corneal epithelium; design; established cell line; feeding schedule; lens; member; programs; repository; research study; response; tissue culture

In response to changing needs of VSRC investigators over the past year, including the addition of two senior faculty members with large programs in this area, Drs. Palczewski and Wensel, the Tissue Culture function was separated from Histology, and has now expanded to include a Hybridoma function. The Tissue Culture and Hybridoma (TCH) Module thus changed from the previous funding period in that Histology is now administratively with Microscopy and Imaging, and we have recruited a Co-Manager to run the Hybridoma facility since August 2006. This revised Module format is designed to provide VSRC investigators with facilities and expert technical assistance for: 1) culture and propagation of established and new cell lines of ocular tissues; and 2) development of monoclonal antibodies to proteins and other antigens of relevance to ocular disease.

在过去的一年里，为了应对VSRC研究人员不断变化的需求，包括增加了两名在该领域有大型项目的高级教员。Palczewski和Wensel，组织培养功能从组织学中分离出来，现在已经扩展到包括杂交瘤功能。因此，组织培养和杂交瘤（TCH）模块与之前的资助期相比发生了变化，因为组织学现在与显微镜和成像一起管理，