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Surface and Interface Mediated Photocoupling Chemistry

表面和界面介导的光耦合化学

基本信息

批准号：
7516586
负责人：
MINGDI YAN
金额：
$ 20.58万
依托单位：
PORTLAND STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-04-01 至 2012-07-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The ability to precisely control surface and interface properties is of paramount importance in applications ranging from adhesion, biomaterials, to medical implants and miniaturized analytical devices. The advance of these fields demands highly sensitive bioanaytical assays and techniques that can offer unprecedented levels of precision and resolution. Surface chemistry is often the bottleneck in the advancement of these techniques. Requirements for surface coupling chemistry include the precise control of the ligand density, orientation and spatial presentation, as well as a matrix that minimizes non-specific interactions. Also highly desirable are coupling chemistries that can accommodate ligands of diverse molecular structures. Techniques that can offer additional benefit of simplicity and versatility are especially attractive in clinical and industrial settings. We have developed a photocoupling chemistry for the covalent attachment of organic molecules on solid substrates. The technique is simple, versatile, and the process can be conveniently modulated by an external light with high precision and spatial resolution. A grand challenge in this photocoupling chemistry is the precise control over the ligand presentation with respect to orientation, conformation, and spatial display. The work described in this proposal is designed to address this challenge. Our hypothesis is that ligand presentation can be controlled by engineering surfaces and interfaces at the molecular level. Using carbohydrates as the model system, we will develop strategies to control the ligand density and the coupling efficiency (AIM 1). The conformation and presentation of the ligand will be addressed by incorporating a physical adsorption process where the ligand conformation will be defined by the interfacial interactions of the ligand with the substrate (AIM 2). Finally, the developed strategies will be employed to couple carbohydrates to nanomaterials. The efficiency of these carbohydrate ligands in capturing bacteria will be studied with regard to the ligand presentation and display (AIM 3). PUBLIC HEALTH RELEVANCE: This project focuses on designing and developing functional surfaces and nanomaterials for specific use in medical devices and diagnostics. The optimized materials will particularly be applied to presentation of carbohydrate structures since glycan-mediated recognition plays a central role in many disease processes, such as bacterial adherence and inflammatory processes. The designed materials will subsequently be applied to nonthrombogenic surfaces for biomaterials and medical devices, and nanomaterial capture of pathogenic cells.

描述（由申请人提供）：精确控制表面和界面特性的能力对于从粘附、生物材料到医疗植入物和小型分析设备的应用至关重要。这些领域的进步需要高度灵敏的生物分析测定和技术，以提供前所未有的精度和分辨率。表面化学往往是这些技术进步的瓶颈。表面耦合化学的要求包括精确控制配体密度、方向和空间呈现，以及最大限度地减少非特异性相互作用的基质。同样非常需要的是能够容纳不同分子结构的配体的偶联化学。能够提供简单性和多功能性的额外优势的技术在临床和工业环境中尤其有吸引力。我们开发了一种光耦合化学，用于将有机分子共价附着在固体基质上。该技术简单、通用，并且可以方便地通过外部光进行调制，具有高精度和空间分辨率。这种光耦合化学的一个巨大挑战是精确控制配体在方向、构象和空间显示方面的呈现。本提案中描述的工作旨在应对这一挑战。我们的假设是配体呈递可以通过分子水平上的工程表面和界面来控制。使用碳水化合物作为模型系统，我们将开发控制配体密度和偶联效率的策略（AIM 1）。配体的构象和呈现将通过结合物理吸附过程来解决，其中配体构象将由配体与基材的界面相互作用来定义（AIM 2）。最后，所开发的策略将用于将碳水化合物与纳米材料偶联。这些碳水化合物配体捕获细菌的效率将在配体呈递和展示方面进行研究（AIM 3）。公共健康相关性：该项目侧重于设计和开发用于医疗设备和诊断的特定用途的功能表面和纳米材料。优化的材料将特别适用于碳水化合物结构的呈现，因为聚糖介导的识别在许多疾病过程中发挥着核心作用，例如细菌粘附和炎症过程。设计的材料随后将应用于生物材料和医疗设备的非血栓形成表面，以及病原细胞的纳米材料捕获。