Chromosomal Rearrangements and Cardiac Candidate Genes

染色体重排和心脏候选基因

• 批准号: 7354823
• 负责人: BEVERLY S EMANUEL
• 金额: $ 47.45万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7354823
• 关键词: 22q; Animal Model; Architecture; Basic Science; Biology; Candidate Disease Gene; Cardiac; Cardiology; Child; Chromosomal Rearrangement; Chromosome abnormality; Chromosomes; Clinical; Computer Simulation; Congenital Heart Defects; Cytogenetic Analysis; Cytogenetics; DNA; DNA Sequence Rearrangement; Databases; Defect; Development; Discipline; Disease; Equilibrium; Ethers; Ethyl Ether; Etiology; Fruit; Funding; Gene Mutation; Genes; Genetic; Genome; Genomics; Genus Capra; Goals; Goat; Heart; Homologous Gene; Human; Human Genome Project; Individual; Infant; Interruption; Karyotype; Lead; Location; Maps; Modeling; Molecular; Molecular Analysis; Molecular Biology; Molecular Cytogenetics; Morphogenesis; Mus; Mutation; Pathway interactions; Patients; Pattern; Pediatric Hospitals; Phenotype; Play; Population; Positioning Attribute; Primed In Situ Labeling; Principal Investigator; Reporting; Research; Resolution; Role; Series; Specificity; Standards of Weights and Measures; Study Subject; Techniques; Tissues; Translocation Breakpoint; United States; Variant; Xenopus; Zebrafish; base; cardiogenesis; cohort; developmental genetics; dosage; interstitial; knockout gene; new technology; novel; positional cloning; programs; research study

The goal in this proposal is to use chromosomal rearrangements (CRs) occurring in children with CHDs as signposts to identify novel genes important in cardiac morphogenesis. The underlying hypothesis of this proposal is that we can identify genes critical to normal cardiac development based upon their location in relation to breakpoints of balanced translocations. Further, we hypothesize that other chromosomal changes, such as additions or interstitial deletions will assist in the identification of additional dosage sensitive genes important to cardiac development. The subjects for this study will be children who present to the SCCOR with heart defects and possibly further associated congenital anomalies. We will utilize the fruits of the Human Genome Project as well as novel technology we are developing to characterize the regions involved in the chromosomal aberrations of SCCOR patients. We hypothesize that these regions harbor genes important to normal cardiac development. We will pursue the molecular identification and analysis of cardiac candidate genes such that mutation studies can be the focus of subsequent research studies. Characterization of the candidate genes will include the delineation of their tissue, spatial and temporal expression patterns in mouse and/or Xenopus. We will isolate or identify homologues of these candidate genes in model organisms. Our specific aims include: 1) identification and characterization of CRs in patients with CHD by high-resolution cytogenetics and molecular cytogenetic analysis; 2) development of PCR-based mapping strategies using the human genomic sequence to identify translocation breakpoints (BPs); 3) Characterization of the genomic DNA from normal chromosomes at the BPs in order to identify mechanisms of rearrangement; 4) identification and characterization of genes disrupted or deleted at the translocation BPs as candidates for early cardiac morphogenesis; and 5) determination of whether mutations in the candidate genes are associated with the cardiac defect in other patients with CHD. This represents a unified program of clinical and basic research. It brings together the disciplines of clinical cardiology, clinical genetics, cytogenetics, molecular biology and developmental genetics to examine the influence of genomic variation on the etiology of CHDs in our quest to discover genes involved in fundamental pathways during early cardiogenesis.

本研究的目的是利用发生在CHDs儿童身上的染色体重排（CRs）作为标志，来识别在心脏形态发生中重要的新基因。这一提议的基本假设是，我们可以根据与平衡易位断点相关的位置识别对正常心脏发育至关重要的基因。此外，我们假设其他染色体变化，如添加或间质缺失，将有助于鉴定对心脏发育重要的其他剂量敏感基因。本研究的对象将是向SCCOR提出心脏缺陷和可能进一步相关的先天性异常的儿童。我们将利用人类基因组计划的成果以及我们正在开发的新技术来描述所涉及的区域