Core--Cell Culture, DNA and Microarray

核心--细胞培养、DNA和微阵列

• 批准号: 7354825
• 负责人: BEVERLY S EMANUEL
• 金额: $ 24.43万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7354825
• 关键词: 22q11; 22q11.2; Blood; Cell Line; Cheek structure; Chromosome Mapping; Congenital Heart Defects; Consultations; Cosmids; Cultured Cells; Cytogenetics; DNA; DNA Microarray Chip; DNA Microarray format; DNA analysis; Equipment; Fluorescent in Situ Hybridization; Genetic; Genotype; Goals; Human; In Situ Hybridization; Individual; Label; Laboratories; Localized; Methods; Molecular Weight; Mutation; Patients; Preparation; Procedures; Reagent; Research Personnel; Resources; Risk; Sampling; Services; Side; Slide; Source; Specimen; Spottings; Swab; Technical Expertise; Technology; Tissues; Title; Training; base; established cell line; genetic analysis; lymphoblastoid cell line; peripheral blood; programs; research study

DESCRIPTION (provided by applicant): 1. Services Offered A. Establish lymphoblastoid cell lines from patients with congenital heart defects. B. Isolate DNA from established cell lines, peripheral blood, blood spots, tissues or cheek swabs. C. Perform FISH to screen for 22q11.2 deletions in ?at-risk" patients. D. Regionally localize newly identified human cDNAs by FISH. E. Provide support for microarray-based experiments. F. Provide genotyping/mutation services for the SCCOR. 2. Functions of the Core Unit as a Resource to the SCCOR Core D will provide the cell culture, DNA isolation, cytogenetic and DNA analysis support for the projects and cores in this SCOR. The change in title to the proposed Cell Culture and DNA Analysis Core reflects the changes in program goals and in the scope and emphasis on new genetic analysis services to be provided by this Core. The routine activities of Core D will include the establishment of cell lines, FISH analysis of DNA specimens for 22q11 deletion, preparation of slides for chromosomal in situ hybridization, gene localization by FISH and isolation of high molecular weight DNA samples from patient samples and cell lines. The also Core will provide investigators access to labeled BAC or cosmid clones for FISH as required. On the DNA analysis side, the Core will provide a centralized source for specialized services, technical expertise and reagents to support the DNA analysis (microarray and genotyping) requirements of the investigators in the SCCOR. The Facility will provide access to equipment and technologies that would be prohibitively expensive for an investigator to possess in an individual laboratory. In addition, the Core Unit Leader and staff will provide training and consultation services to the SCCOR investigators and provide training to young and mid-level investigators in genetic laboratory methods and procedures. The following sections briefly summarize the Core functions.

描述（由申请人提供）：