Core--Cell Culture, DNA and Microarray

核心--细胞培养、DNA和微阵列

• 批准号: 7354825
• 负责人: BEVERLY S EMANUEL
• 金额: $ 24.43万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7354825
• 关键词: 22q11; 22q11.2; Blood; Cell Line; Cheek structure; Chromosome Mapping; Congenital Heart Defects; Consultations; Cosmids; Cultured Cells; Cytogenetics; DNA; DNA Microarray Chip; DNA Microarray format; DNA analysis; Equipment; Fluorescent in Situ Hybridization; Genetic; Genotype; Goals; Human; In Situ Hybridization; Individual; Label; Laboratories; Localized; Methods; Molecular Weight; Mutation; Patients; Preparation; Procedures; Reagent; Research Personnel; Resources; Risk; Sampling; Services; Side; Slide; Source; Specimen; Spottings; Swab; Technical Expertise; Technology; Tissues; Title; Training; base; established cell line; genetic analysis; lymphoblastoid cell line; peripheral blood; programs; research study

DESCRIPTION (provided by applicant): 1. Services Offered A. Establish lymphoblastoid cell lines from patients with congenital heart defects. B. Isolate DNA from established cell lines, peripheral blood, blood spots, tissues or cheek swabs. C. Perform FISH to screen for 22q11.2 deletions in ?at-risk" patients. D. Regionally localize newly identified human cDNAs by FISH. E. Provide support for microarray-based experiments. F. Provide genotyping/mutation services for the SCCOR. 2. Functions of the Core Unit as a Resource to the SCCOR Core D will provide the cell culture, DNA isolation, cytogenetic and DNA analysis support for the projects and cores in this SCOR. The change in title to the proposed Cell Culture and DNA Analysis Core reflects the changes in program goals and in the scope and emphasis on new genetic analysis services to be provided by this Core. The routine activities of Core D will include the establishment of cell lines, FISH analysis of DNA specimens for 22q11 deletion, preparation of slides for chromosomal in situ hybridization, gene localization by FISH and isolation of high molecular weight DNA samples from patient samples and cell lines. The also Core will provide investigators access to labeled BAC or cosmid clones for FISH as required. On the DNA analysis side, the Core will provide a centralized source for specialized services, technical expertise and reagents to support the DNA analysis (microarray and genotyping) requirements of the investigators in the SCCOR. The Facility will provide access to equipment and technologies that would be prohibitively expensive for an investigator to possess in an individual laboratory. In addition, the Core Unit Leader and staff will provide training and consultation services to the SCCOR investigators and provide training to young and mid-level investigators in genetic laboratory methods and procedures. The following sections briefly summarize the Core functions.

描述（由申请人提供）： 1.提供的服务 A.建立先天性心脏病患者的淋巴母细胞系。 B。从已建立的细胞系、外周血、血斑、组织或颊拭子中分离DNA。 C.进行FISH筛查22q11.2缺失？“高危”患者。 D.通过FISH对新鉴定的人cDNA进行区域定位。 E.支持基于微阵列的实验。 F.为SCCOR提供基因分型/突变服务。 2.核心股作为SCCOR资源的职能 核心D将提供细胞培养、DNA分离、细胞遗传学和DNA分析支持， 项目和核心在这个SCOR。拟议的细胞培养和DNA分析核心项目名称的变更反映了该核心项目提供的新遗传分析服务的项目目标、范围和重点的变化。核心D的常规活动将包括建立细胞系、对DNA标本进行22 q11缺失的FISH分析、制备用于染色体原位杂交的载玻片、通过FISH进行基因定位以及从患者样本和细胞系中分离高分子量DNA样本。此外，Core将根据需要为研究者提供标记的BAC或粘粒克隆用于FISH。在DNA分析方面，核心将提供专门服务、技术专长和试剂的集中来源，以支持SCCOR研究人员的DNA分析（微阵列和基因分型）要求。该设施将提供设备和技术，这些设备和技术对调查人员来说过于昂贵，无法在单个实验室中使用。此外，核心股负责人和工作人员将向SCCOR调查人员提供培训和咨询服务，并向年轻和中级调查人员提供遗传实验室方法和程序方面的培训。以下各节简要总结了核心功能。