ANIMAL BARRIER SHARED RESOURCE

动物屏障共享资源

基本信息

批准号：
7506828
负责人：
LAWRENCE H HERBST
金额：
$ 19.85万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-25 至 2012-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7506828
关键词：
Address Albert Einstein Cancer Center Alleles Animal Experimentation Animal Husbandry Animal Technicians Animal Welfare Animals Antineoplastic Agents Area Arts Behavioral Biopsy Breeding Cancer Center Cancer Center Support Grant Caring Censuses Characteristics Comprehensive Health Care Condition Daily Diagnostic Services Doctor of Philosophy Doctor of Veterinary Medicine Environment Faculty Film Floor Genetic Genetic Predisposition to Disease Genotype Gnotobiotic Goals Grant Growth Health Histopathology House mice Housing Implant Individual Infectious Agent Institutes Laboratory Animals Mission Modeling Monitor Mus Mutation Neoplasm Metastasis Neoplasms Numbers Pathologist Pathology Phenotype Price Process Quarantine Research Research Personnel Resource Sharing Rodent Services Site Visit Standards of Weights and Measures Technology Testing Time Tissues Training Transgenes Tumor Tissue Veterinarians angiogenesis animal care animal resource germ free condition improved novel programs quality assurance research study translational medicine tumor growth tumor xenograft

项目摘要

The Animal Barrier Shared Resource (ABSR) supports the mission of the AECC by providing an environment in which the highest quality of animal research studies can be conducted while maintaining the highest standards of animal care and animal welfare. A major objective of the ABSR is to maintain the health quality of barrier housed specific pathogen-free (SPF) mice, keeping them free of adventitious murine infectious agents that could disrupt breeding or confound research results. Barrier housing is provided for SPF mice while permitting supervised access for investigators. AECC investigators maintain breeding colonies either to expand novel strains bearing transgene or targeted mutations from genetically-manipulated founder animals, or to recombine several specific alleles from different genetic loci into a limited set of genotypes on a single strain background. Frequent convenient access is crucial for investigators so that they may set up matings, collect tissue biopsies from successive litters for genotype determination, and observe animals for physical and behavioral characteristics (phenotype), specifically, their propensity to develop neoplasia. In addition, many AECC investigators use specific pathogen-free rodents that either have a genetic predisposition to develop spontaneous neoplasia or are immunodeficient and tolerate tumor xenografts as models to study specific processes such as angiogenesis and metastasis and for testing antineoplastic therapies. These investigators must have frequent access to their animals to implant tumor tissues, monitor tumor growth, administer various treatments, and collect tissue biopsies. Animal husbandry services, veterinary care, and comprehensive rodent quality assurance and quarantine programs are provided to the AECC by the Institute of Animal Studies (IAS). The IAS consists of three faculty Laboratory Animal Veterinarians (including the Director), three Veterinary Technicians, a staff of approximately 40 Animal Caretakers, a Facilities Manager, two Husbandry Supervisors, as well as office administrative staff. In addition to providing veterinary care, the IAS veterinarians also provide oversight of the animal care and use program, monitor regulatory compliance, and conduct small-group and individual training sessions for investigators. Pathology diagnostic services are provided by a Veterinary Pathologist who also directs the Histopathology Shared Resource.

动物屏障共享资源（absr）通过提供AECC的使命可以在维持动物研究的最高质量研究的环境中保持动物保健和动物福利的最高标准。 abr的主要目标是维护障碍物的健康质量容纳特定的无病原体（SPF）小鼠，使它们无偶然的鼠可能破坏繁殖或混淆研究结果的传染性药物。提供了障碍物 SPF小鼠在允许调查人员的监督访问权限的同时。 AECC调查人员保持育种菌落要么从遗传操纵中扩展带有转基因的新型菌株或靶向突变创始人的动物，或将不同遗传基因座的几个特定等位基因重组为有限的一组单个应变背景上的基因型。频繁的方便访问对于调查人员至关重要他们可能会设置总体，从连续的垃圾中收集组织活检以确定基因型，并观察动物的身体和行为特征（表型），特别是它们的倾向发展肿瘤。此外，许多AECC研究人员使用特定的无病原体啮齿动物具有发展自发肿瘤或免疫缺陷且耐受性肿瘤的遗传易感性异种移植物作为研究特定过程（例如血管生成和转移）的模型以及测试抗肿瘤的模型疗法。这些研究者必须经常进入动物植入肿瘤组织，监测肿瘤生长，进行各种治疗，并收集组织活检。畜牧业服务，兽医护理以及全面的啮齿动物质量保证和隔离计划动物研究所（IAS）向AECC提供。 IAS由三个教师实验室组成动物兽医（包括导演），三名兽医技术人员，约40名员工动物看护人，设施经理，两名饲养主管以及办公室行政人员。除了提供兽医护理外，IAS兽医还提供动物护理和使用计划，监控法规合规性，并进行小组和个人培训课程调查人员。病理诊断服务由兽医病理学家提供组织病理学共享资源。