Molecular Biology

分子生物学

• 批准号: 7278946
• 负责人: David James Looney
• 金额: $ 24.01万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7278946
• 关键词: Acquired Immunodeficiency Syndrome; Anti-Retroviral Agents; Applications Grants; Base Sequence; Bioinformatics; Biological Assay; California; Cell Line; Client; Clinical; Cloning; Computer software; Condition; Custom; DNA Sequence; Databases; Detection; Development; Disease; Facility Construction Funding Category; Feline Immunodeficiency Virus; Feline Leukemia Virus; Foundations; Gene Silencing; Genes; Genetic Transcription; Genomics; Genotype; Grant; HIV; HIV-1; HIV-2; Healthcare Systems; Hepatitis C virus; Human Herpesvirus 4; Human Herpesvirus 8; Immunologic Deficiency Syndromes; Institution; Kaposi Sarcoma; Kinetics; Lentivirus Vector; Maps; Medical Research; Mentors; MicroRNAs; Molecular Biology; Molecular Biology Techniques; Molecular Biology, Other; Molecular Virology; Murine leukemia virus; Nucleic acid sequencing; Pathogenesis; Plasma; Plasmids; Polymerase Chain Reaction; Preparation; Publications; Purpose; RNA; RNA Interference; Reagent; Research; Research Infrastructure; Research Institute; Research Personnel; Resistance; Resources; Retroviridae; SIV; Sampling; Satellite Viruses; Services; Spottings; Students; Subfamily lentivirinae; Support of Research; Techniques; Technology; Testing; Time; Training; Training Support; Universities; Variant; Veterans; Viral; Virus; Virus Replication; Work; base; commercial application; cost; design; drug metabolism; expression vector; immunoprophylaxis; interest; knockout gene; mecarzole; medical specialties; novel strategies; small hairpin RNA; software development; tissue culture; vector

The Molecular Biology Core is designed to expedite access to basic molecular biology techniques, resources, and training needed by investigators exploring problems in HIV replication, pathogenesis, therapy, and immunoprophylaxis. A particular emphasis, is the support of developmental projects and investigators just entering the field of HIV research. The Core has four specific objectives: (1) The Core supports research requiring RNA and DMA sequence information. Low-cost, rapid, accurate DNA sequencing is provided, along with special expertise in sequencing of HIV-1, HIV-2, SIV, and other lentiviruses. Training in preparation and sequencing for custom applications, and optimization of sequencing under specialized conditions for requested projects is supported. (2) The Core supports other aspects of molecular biology research. This includes amplification and hybridization techniques for detection and quantification (plasma, tissue and culture samples) for AIDS-related viruses and vectors including HIV-1, HIV-2, SIV, EBV, KSHV, and HCV viruses, as well as MuLV-, HIV-1-, HIV-2-, and FIV-based vectors. (3) The Core provides support for gene-silencing technology. This includes siRNA design, synthesis, and vector construction, as well as lentiviral vectors for siRNA/shRNA expression. (4) The Core supports other aspects of molecular biology needed for HIV research. This includes distribution of specialty reagents (primers, plasmids, maps, other) and assistance with design of projects involving PCR and/or cloning. The Core also supports training seminars, and provides one-on-one training for fellows and students in sequencing techniques, methods, and techniques related to miRNA and siRNA, and QC and kinetic real-time PCR. Mentoring is provided to developmental investigators. In addition, the Core assists in acquisition, support, and administration of computational infrastructure, and facilitates access to third-party molecular biology software needed by HIV/AIDS investigators. Past and ongoing molecular biology support of UCSD research efforts illustrates the Core's usefulness. Since the last competitive renewal in 2002, work performed by the Molecular Biology Core has been identified by 111 different investigators who have used the Core in generating 92 publications and 40 new or renewal grant applications. Research efforts supported by the Core have increased our understanding of viral resistance, viral variation and compartmentalization, the immunopathogenesis of disease, and new approaches for treatment and immunoprophylaxis. In summary, the Molecular Biology Core is a valuable, productive, and popular core resource for the UCSD CFAR.

分子生物学核心旨在加快获得基本分子生物学技术， 研究人员探索艾滋病毒复制、发病机制、 治疗和免疫预防。特别强调的是对发展项目的支持 研究人员刚刚进入艾滋病毒研究领域。 核心有四个具体目标：(1) 核心支持需要 RNA 和 DMA 序列的研究 信息。提供低成本、快速、准确的 DNA 测序，以及以下领域的特殊专业知识： HIV-1、HIV-2、SIV 和其他慢病毒的测序。定制准备和排序培训 应用程序，并在特定条件下针对所请求的项目优化排序 支持。 (2) 核心支持分子生物学研究的其他方面。这包括放大 用于检测和定量（血浆、组织和培养样品）的杂交技术 艾滋病相关病毒和载体，包括 HIV-1、HIV-2、SIV、EBV、KSHV 和 HCV 病毒，以及 基于 MuLV、HIV-1、HIV-2 和 FIV 的载体。 (3) Core为基因沉默提供支持 技术。这包括 siRNA 设计、合成和载体构建，以及慢病毒载体 siRNA/shRNA 表达。 (4) 核心支持 HIV 所需的分子生物学的其他方面 研究。这包括专业试剂（引物、质粒、图谱等）的分发和协助 涉及 PCR 和/或克隆的项目设计。该核心还支持培训研讨会，以及 为研究员和学生提供测序技术、方法和技术方面的一对一培训 与 miRNA 和 siRNA 以及 QC 和动态实时 PCR 相关。为发展提供指导 调查人员。此外，核心还协助获取、支持和管理计算 基础设施，并促进获取艾滋病毒/艾滋病所需的第三方分子生物学软件 调查人员。 加州大学圣地亚哥分校研究工作过去和正在进行的分子生物学支持说明了核心的有用性。 自 2002 年上次竞争性更新以来，分子生物学核心所做的工作一直在 由 111 名不同的研究人员确定，他们使用该核心生成了 92 份出版物和 40 份新出版物 或续展补助金申请。核心支持的研究工作增加了我们对 病毒耐药性、病毒变异和区室化、疾病的免疫发病机制以及新的 治疗和免疫预防的方法。 总之，分子生物学核心对于 UCSD 来说是一个有价值的、富有成效的、受欢迎的核心资源 恒虚警。