CORE--MOLECULAR BIOLOGY

核心--分子生物学

• 批准号: 6500679
• 负责人: David James Looney
• 金额: $ 18.05万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2002-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6500679
• 关键词: AIDS; AIDS therapy; HIV infections; biomedical facility; chemokine; computer assisted sequence analysis; cytokine receptors; genetic library; genotype; human herpesvirus 8; human immunodeficiency virus; messenger RNA; microorganism resource /registry; molecular biology; nucleic acid sequence; plasmids; polymerase chain reaction; virus RNA; virus cytopathogenic effect; virus genetics; virus replication

The overall purpose of the Molecular Biology Core (Core C) is to provide efficient shared access to basic molecular biology services for investigators for need to apply a variety of molecular techniques to problems involving HIV replication, pathogenesis, therapy, and immunoprophylaxis of HIV infection, with particular emphasis on support of developmental projects. Specific objectives of Core C include: [1] To provide small and large- scale, cost efficient DNA sequencing for CFAR laboratories, [2] To provide accurate message analysis services to core members, [3] To manage a plasmid library, providing large scale preparation of full-length HIV-1 and HIV-2 viral clones, and [4] To manage a PCR primer library for the efficient distribution of primers shared among investigators. Ongoing Molecular Biology Core support of UCSD research efforts clearly illustrates its need and usefulness. Large sequencing efforts supported include sequencing of Coccididiomycosis inmitis, Aspergillus fumigatus, and Toxoplasma gondii, envelope fragments from pediatric and adult CNS sources ongoing analysis pol mutations induced by treatment with protease inhibitors, and analysis of sequence variation in an HIV-2 M. nemestrina vaccine model. Such efforts are crucial to our understanding of viral resistance, and the meaning of viral variation. The recent introduction of quantitative RT PCR assays for chemokine receptor and beta-chemokine messages by Core C has supported research on HIV induced apoptosis, differential co-receptor usage by HIV strains, the role of co-receptors in nef action, and the use of chemokine receptors by other lentiviruses. The Molecular Biology Core plasmid library has facilitated early work on DNA vaccines for HIV, investigation of nef enhancement of infectivity, determination of HIV genes which contribute to apoptosis, work on bacterial vectors for AIDS vaccines. Primers designed by the core have proven valuable to the construction of new and very useful reporter cell lines bearing CCR5 receptors. The proposed Molecular Biology Core (Core C) represents an extension of a model service core which has been thoroughly tested and proven to be a productive, cost-efficient and useful core resource in the existing UCSD Center for AIDS Research.

分子生物学核心（核心C）的总体目的是提供 高效共享基础分子生物学服务， 研究人员需要应用各种分子技术， 涉及HIV复制、发病机制、治疗和 艾滋病毒感染免疫预防，特别强调支持 发展项目。 核心C的具体目标包括：[1]提供小型和大型- 为CFAR实验室提供规模化、经济高效的DNA测序，[2] 为核心会员提供准确的消息分析服务，[3] 质粒文库，提供全长HIV-1的大规模制备 和HIV-2病毒克隆，以及[4]为了管理用于 研究者之间共享引物的有效分配。 持续的分子生物学明确支持加州大学圣地亚哥分校研究工作 说明了它的必要性和实用性。支持大型测序工作 包括对球虫病、烟曲霉 和弓形虫，来自儿童和成人CNS的包膜片段 来源正在进行的分析蛋白酶处理诱导的pol突变 抑制剂，并分析HIV-2 M中的序列变异。nemestrina 疫苗模型这些努力对于我们理解病毒的 以及病毒变异的意义。最近采用 趋化因子受体和β-趋化因子的定量RT PCR分析 核心C的信息支持了HIV诱导细胞凋亡的研究， HIV毒株对共受体的不同使用，共受体在 nef作用，以及其他慢病毒对趋化因子受体的使用。的 分子生物学核心质粒文库促进了DNA的早期工作 艾滋病毒疫苗，对感染性增强的研究， 确定艾滋病毒基因，有助于细胞凋亡，工作 艾滋病疫苗的细菌载体。由核心设计的引物具有 被证明对构建新的和非常有用的报告细胞有价值 携带CCR 5受体的细胞系。 拟议的分子生物学核心（核心C）代表了一个 模型服务核心，已经过全面测试，并证明是一个 现有UCSD中的生产性，成本效益和有用的核心资源 艾滋病研究中心。