MOLECULAR PATHOPHYSIOLOGY CORE

核心D： 该中心的总体目标是提供最先进的病理和生理专家支持 基础科学和临床项目。肺气肿和慢性支气管炎涉及结构改变， 可以从形态上进行评估。事实上，肺病理是诊断慢性阻塞性肺疾病的金标准。 肺气肿。此外，我们独一无二地适合将高质量的形态和生理 肺组织的评估，结合分子和细胞病理过程的询问 与慢性阻塞性肺病有关。我们的核心已经发展了最先进的肺部显微成像和计算机辅助 形态测量和小动物肺功能测试，使我们能够整合结构异常 以及它们对肺部生理的影响。PI、Tuder博士、Co-Pi和Mitzner有很长的记录 科学协作，这将有助于核心的成功。 我们的目标是提供高质量的组织处理，准确的表型表征 肺泡和细支气管结构，以及最先进的工具来调查和验证潜力 疾病的标志物和病理相关的分子，可能在影响 薄壁组织！慢性阻塞性肺疾病的肺损伤、细支气管壁重构和结构改变。 为实现这些目标，我们提出了以下战略： 1)协助研究人员进行以牙槽骨为研究对象的实验设计 和呼吸道结构(项目1、2和4)。2)在实验模型上进行肺功能测试 肺气肿(项目1、2、3和4)。3)进行图案的免疫组织化学表征 被确认为对实验重要的标志物和相关蛋白质的肺分布 项目1、2、3、4和5.4)执行针对转录本在肺中的定位的原位杂交 (项目1、2、3和4)。5)。将蛋白质和转录数据与形态变化相关联 存在于光镜水平(项目1-4)。

CORE D: The overall goal of the core is to provide state of the art pathological and physiological expert support to basic science and clinical projects. Emphysema and chronic bronchitis involve structural alterations, which can be assessed morphologically. In fact, lung pathology is the gold standardfor the diagnosis of emphysema. Furthermore, we are uniquely fitted to integrate high quality morphological and physiological assessment of lung tissue, coupled with the interrogation of molecular and cellular pathological processes involved in COPD. Our core has developed state of the art lung microscopic imaging and computer-assisted morphometry, and small animal pulmonary function testing, allowing us to integrate structural abnormalities and their impact on lung physiology. The PI, Dr. Tuder, and Co-Pi and Mitzner have a long record of scientific collaboration, which will be instrumental for the success of the core. It is our goal to provide high quality tissue processing, accurate phenotypic characterization of alveolar and bronchiolar structure, and state-of-the art tools to investigate and validate potential markers of disease and pathogenetically relevant molecules that may have a role on the impact on parenchyma! lung injury, bronchiolar remodeling, andstructural alterations in COPD. We propose the following Strategies to accomplish these goals: 1) To assist the investigators in all projects in the experimental design aimed at investigation of alveolar and airway structure (Projects 1,2, and 4). 2) Perform pulmonary function testing in experimental models of emphysema (Projects 1,2, 3 and 4). 3) To perform immunohistochemical characterization of the pattern of lung distribution of markers and relevant proteins identified as important to the experiments outlined in Projects 1, 2, 3, 4, and 5. 4) To perform in situ hybridization aimed at the transcript localization in lungs (Projects 1, 2, 3, and 4). 5). To correlate the protein and transcriptional data with morphological alterations present at the light microscopic level (Projects 1-4).