MOLECULAR PATHOPHYSIOLOGY CORE

分子病理生理学核心

• 批准号: 7499947
• 负责人: Rubin M. Tuder
• 金额: $ 27.16万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-14 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7499947
• 关键词: Alveolar; Animals; Apoptosis; Arts; Autopsy; Basic Science; Biological Assay; Biopsy; Cardiopulmonary; Cataloging; Catalogs; Chronic Bronchitis; Chronic Obstructive Airway Disease; Clinical; Clinical Research; Collaborations; Complex; Computer Assisted; Coupled; Data; Diagnosis; Disease Marker; Distal; Documentation; Event; Excision; Experimental Designs; Experimental Models; Functional disorder; Genetic Transcription; Genomics; Goals; Gold; Human; Image; Immunofluorescence Immunologic; In Situ Hybridization; Inflammation; Investigation; Laboratories; Learning; Light; Lung; Lung Neoplasms; Lung diseases; Microscopic; Molecular; Morphology; Nature; Oxidative Stress; Pathologic Processes; Pathology; Patients; Pattern; Physiological; Physiology; Process; Proteins; Pulmonary Emphysema; Pulmonary function tests; Research Personnel; Rodent; Role; Structure; Structure of parenchyma of lung; Terminal Bronchiole; Time; Transcript; Translations; Western Blotting; alveolar destruction; base; cell type; diagnosis standard; lung injury; morphometry; programs; research study; success; tissue processing; tool

CORE D: The overall goal of the core is to provide state of the art pathological and physiological expert support to basic science and clinical projects. Emphysema and chronic bronchitis involve structural alterations, which can be assessed morphologically. In fact, lung pathology is the gold standardfor the diagnosis of emphysema. Furthermore, we are uniquely fitted to integrate high quality morphological and physiological assessment of lung tissue, coupled with the interrogation of molecular and cellular pathological processes involved in COPD. Our core has developed state of the art lung microscopic imaging and computer-assisted morphometry, and small animal pulmonary function testing, allowing us to integrate structural abnormalities and their impact on lung physiology. The PI, Dr. Tuder, and Co-Pi and Mitzner have a long record of scientific collaboration, which will be instrumental for the success of the core. It is our goal to provide high quality tissue processing, accurate phenotypic characterization of alveolar and bronchiolar structure, and state-of-the art tools to investigate and validate potential markers of disease and pathogenetically relevant molecules that may have a role on the impact on parenchyma! lung injury, bronchiolar remodeling, andstructural alterations in COPD. We propose the following Strategies to accomplish these goals: 1) To assist the investigators in all projects in the experimental design aimed at investigation of alveolar and airway structure (Projects 1,2, and 4). 2) Perform pulmonary function testing in experimental models of emphysema (Projects 1,2, 3 and 4). 3) To perform immunohistochemical characterization of the pattern of lung distribution of markers and relevant proteins identified as important to the experiments outlined in Projects 1, 2, 3, 4, and 5. 4) To perform in situ hybridization aimed at the transcript localization in lungs (Projects 1, 2, 3, and 4). 5). To correlate the protein and transcriptional data with morphological alterations present at the light microscopic level (Projects 1-4).

核心D： 核心的总体目标是提供最先进的病理和生理专家支持， 基础科学和临床项目。肺气肿和慢性支气管炎涉及结构改变， 可以从形态学上进行评估。事实上，肺部病理学是诊断的金标准事实上，肺部病理学是诊断的金标准 肺气肿此外，我们是唯一适合整合高品质的形态和生理 肺组织评估，结合分子和细胞病理过程的询问 参与COPD。我们的核心已经开发出最先进的肺部显微成像和计算机辅助 形态测量和小动物肺功能测试，使我们能够整合结构异常 以及它们对肺部生理的影响。PI，Tuder博士，Co-Pi和Mitzner有很长的记录， 科学合作，这将有助于核心的成功。 我们的目标是提供高质量的组织处理，准确的表型表征， 肺泡和细支气管结构，以及最先进的工具，以调查和验证潜在的 疾病的标志物和致病相关的分子，可能有一个作用， 薄壁组织！COPD中的肺损伤、细支气管重塑和结构改变。 我们提出以下战略来实现这些目标： 1)协助研究者在所有项目的实验设计，旨在调查肺泡 和气道结构（项目1、2和4）。2)在实验模型中进行肺功能测试 肺气肿（项目1、2、3和4）。3)进行免疫组织化学表征的模式 肺分布的标记物和相关蛋白质被鉴定为对在 项目1、2、3、4和5。4)进行原位杂交，目的是在肺中定位转录本 （项目1、2、3和4）。5）。将蛋白质和转录数据与形态学改变相关联 在光学显微镜水平上存在（项目1-4）。