CORE--Quantitative Morphology

核心--定量形态学

• 批准号: 7490653
• 负责人: PATRICK R HOF
• 金额: $ 17.92万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7490653
• 关键词: 3-Dimensional; Amygdaloid structure; Anatomy; Atlases; Cells; Chromosome Pairing; Computer information processing; Data; Data Set; Dendrites; Development; Electron Microscopy; Functional Magnetic Resonance Imaging; Human; Image; Life; Link; Magnetic Resonance; Methods; Microscopy; Modification; Molecular; Morphology; Neurons; Nuclear; Positron-Emission Tomography; Property; Resolution; Spatial Distribution; Synapses; Technology; Variant; Vertebral column; design; digital; interest; lucifer yellow; mathematical model; member; particle; simulation; tool

The Quantitative Morphology Core (QMC) provides technology supports to the members of the Conte Center. The QMC has developed tools to quantify morphologic changes in spines and synapses at the confocal , multiphoton, and electron microscopy levels. These methods are developed to provide to provide the necessary degree of resolution to quantify plasticity-related changes in molecular constituents of synapses, a critical step to link such modifications to information processing. The QMC has developed applications designed to increase the throughput and accuracy, and reduce bias of quantitative analysis of immunogold electron microscopy of synapses as well as new tools to image Lucifer Yellow-injected neurons at high resolution from automatized acquisition of entire cells using confocal and multiphoton data.. These tools involve deconvolution, smoothing, automatic tiling, and 3-dimensional rendition with high level of detail and capacity for morphometric analysis of dendrites at the spine level of resolution. We are also performing mathematical modeling of neuronal complexity and biophysical simulation of the properties of the reconstructed neurons. The QMC was also involved in the development of a new stereologic probe to assess the 3-dimensional spatial distribution of particles in a volume of interest. Recently, the QMC has begun producing high resolution magnetic resonance microscopy (MRM; 9.4 T) datasets of the human amygdala to provide users with a high level of detail digital atlas of the human amygdala to be used as a reference guide for fMRI and PET analyses of live subjects as well as anatomic reference for amygdala nuclear parcellation. These MRM data will also inform the users on the degree of anatomic variation that occurs in the human amygdala.

定量形态学核心（QMC）为Conte中心的成员提供技术支持。QMC已经开发了工具，在共聚焦，多光子和电子显微镜水平上量化脊柱和突触的形态学变化。开发这些方法以提供必要程度的分辨率来量化突触的分子成分中的可塑性相关变化，这是将这种修饰与信息处理联系起来的关键步骤。QMC已经开发了旨在提高吞吐量和准确性的应用程序，并减少突触免疫金电子显微镜定量分析的偏差，以及使用共聚焦和多光子数据自动采集整个细胞以高分辨率对荧光黄注射神经元进行成像的新工具。这些工具涉及去卷积，平滑，自动平铺，和三维渲染与高层次的细节和能力的树突形态分析在脊柱水平的分辨率。我们还进行神经元复杂性的数学建模和重建神经元特性的生物物理模拟。QMC还参与了一种新的体视学探针的开发，以评估感兴趣体积中颗粒的三维空间分布。最近，QMC已经开始生产高分辨率磁共振显微镜（MRM; 9.4 T）的人类杏仁核数据集，为用户提供高层次的详细数字图谱的人类杏仁核被用作fMRI和PET分析的参考指南，以及解剖参考杏仁核parcellation。这些MRM数据还将告知用户在人类杏仁核中发生的解剖变异的程度。