权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Tumor perfusion in small animals with tomographic digital subtraction angiography

断层数字减影血管造影对小动物的肿瘤灌注

基本信息

批准号：
7382461
负责人：
CRISTIAN T BADEA
金额：
$ 15.6万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-03-12 至 2009-02-28
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The in-vivo imaging of tumor vasculature in rodents can provide invaluable information about tumor development and treatment efficacy. Imaging in vivo fine micro-vascular structure and function presents a challenge to existing small animal imaging techniques including micro-CT and micro-MRI due to combined requirements for high spatial and temporal resolution. We propose the use of Tomographic Digital Subtraction Angiography (TDSA) for estimation of tumor perfusion in small animals. TDSA combines the advantages of high temporal resolution of digital subtraction angiography (DSA) and high spatial resolution of micro-CT. TDSA augments projection imaging methods such as DSA by providing three-dimensional information using tomosynthesis and CT reconstruction algorithms. TDSA approach is based on the novel paradigm that the same time density curves can be reproduced in a number of consecutive injections of microL volumes of contrast at a series of different angles of rotation. We will build a TDSA system with two tubes/detectors to provide data for 4D imaging with a temporal resolution on the order of 100 ms and spatial resolution ranging from 100 microns to 1 mm. This represents a ten fold increase in temporal resolution and at least two times higher resolution than those achieved by the state of the art pre-clinical micro-CT systems used in perfusion imaging in small animals. The capabilities of TDSA will be established in studies on tumor physiology in both rats and mice. TDSA perfusion measures will be calibrated and validated using a dynamic phantom and techniques exploiting microspheres and vascular staining. We will integrate TDSA with conventional micro-CT to provide a standardized protocol for characterization of tumor perfusion, permeability and 3D vascular architecture, using a combination of conventional and blood pool iodinated contrast agents. TDSA will provide the 4D functional imaging method capable of achieving the very high temporal and spatial resolution required for studies on tumor physiology and evaluation of anti-angiogenic drugs on small animals.

描述（由申请方提供）：啮齿动物肿瘤血管系统的体内成像可提供有关肿瘤发展和治疗疗效的宝贵信息。由于对高空间和时间分辨率的组合要求，对体内精细微血管结构和功能进行成像对现有的小动物成像技术（包括微CT和微MRI）提出了挑战。我们建议使用断层数字减影血管造影（TDSA）估计肿瘤灌注在小动物。TDSA结合了数字减影血管造影（DSA）的高时间分辨率和微型CT的高空间分辨率的优点。TDSA通过使用断层合成和CT重建算法提供三维信息来增强DSA等投影成像方法。TDSA方法是基于新的范例，相同的时间密度曲线可以在一系列不同的旋转角度的microL体积的对比剂的连续注射的数量再现。我们将建立一个TDSA系统与两个管/探测器，以提供数据的4D成像的时间分辨率为100毫秒的顺序和空间分辨率范围从100微米到1毫米。这代表了一个10倍的时间分辨率增加和至少两倍的分辨率比在小动物的灌注成像中使用的最先进的临床前微型CT系统所取得的。将在大鼠和小鼠的肿瘤生理学研究中确定TDSA的能力。将使用动态体模和利用微球和血管染色的技术校准和验证TDSA灌注测量。我们将整合经皮数字减影血管造影与传统的micro-CT，提供一个标准化的协议，肿瘤灌注，渗透性和三维血管结构的表征，使用常规和血池碘造影剂的组合。TDSA将提供4D功能成像方法，能够实现肿瘤生理学研究和小动物抗血管生成药物评价所需的非常高的时间和空间分辨率。