Operant Behavioral Instrument for Measuring Pain in Rodents

用于测量啮齿动物疼痛的操作行为仪器

• 批准号: 7482752
• 负责人: RICHARD H MILLS
• 金额: $ 19.76万
• 依托单位: STOELTING COMPANY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-15 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7482752
• 关键词: Absence of pain sensation; Address; Advanced Development; Affect; American; Analgesics; Animal Model; Animals; Applied Research; Automation; Behavior; Behavior assessment; Behavioral; Behavioral Assay; Biological Assay; Biological Models; Brain Stem; Caring; Characteristics; Clinical; Computer software; Condition; Conflict (Psychology); Data; Development; Devices; Disease; Engineering; Epidemic; Equipment; Face; Figs - dietary; Future; Goals; Grooming; Inflammation; Investigation; Knockout Mice; Lead; Light; Literature; Manufactured Supplies; Measures; Mechanics; Mediating; Metals; Methods; Milk; Mind; Modeling; Molecular; Mus; Nociception; Orofacial Pain; Outcome; Pain; Pain Disorder; Pain Measurement; Pain Research; Pharmaceutical Preparations; Phase; Pliability; Process; Public Health; Publishing; Range; Rattus; Reflex action; Research; Research Personnel; Response to stimulus physiology; Rewards; Rodent; Sales; Scientist; Sensory; Societies; Stimulus; Study models; Surface; System; Techniques; Testing; Thermal Hyperalgesias; Time; Translating; Translations; Vendor; Work; allodynia; base; behavior test; bench to bedside; chronic pain; clinically relevant; commercialization; cost; data acquisition; design; experience; improved; in vivo; inflammatory pain; innovation; instrument; novel; prototype; response; size; translational approach; user-friendly

DESCRIPTION (provided by applicant): Chronic pain remains a major burden on society, significantly affecting millions of people and costing the populace billions annually. Despite major advances made to our understanding of pain, very few novel therapies have successfully translated to clinical care. A particular shortfall in this translation is due to the lack of a reliable, high-throughput, commercially available in vivo assay in rodents, particularly in mice. To address this deficit, we propose an innovative, automated, operant behavioral approach for assessing pain in rodents, with our long-range goal being the establishment of an operant model system that will quickly, systematically, and effectively evaluate pain. The central hypothesis is that operant assays can provide a sensitive, reliable, and clinically relevant way to evaluate sensory and nociceptive processing. One specific aim will be investigated: to evaluate a mouse operant model of thermal hyperalgesia and allodynia as compared to the rat operant model for studying pain. We will test this aim using an extremely innovative, automated, high-throughput approach for testing pain and analgesia in mice and rats. This includes using a reward-conflict strategy for assessing thermal pain. The rationale for the proposed research is that use of these operant assays in rats and mice to evaluate pain will provide a better method for future investigation of the mechanisms underlying pain disorders. This study explores an innovative approach for evaluating animal models of pain and would significantly advance the development of new treatments by providing a quick and reliable pain testing system using rodents. The product and process to be developed is an automated, user-friendly, investigator-independent, high-throughput, in vivo pain and analgesic testing system. We are particularly well-suited to complete this body of work, given the pairing of Stoelting Company's expertise in developing the product and Drs. Neubert and Caudle's pain-research experience. This line of research is significant because it explores a novel, high-throughput method to assess pain and pain relief following analgesic administration. If this line of research is successful, the result will have a significant impact on the way new pain-relieving therapies are screened and evaluated, ultimately allowing for a great reduction in the time for new drugs to become available clinically.

描述（由申请人提供）：慢性疼痛仍然是社会的主要负担，严重影响数百万人，每年给民众造成数十亿美元的损失。尽管我们对疼痛的理解取得了重大进展，但很少有新疗法能够成功转化为临床护理。这种翻译的一个特别不足之处是由于缺乏可靠的、高通量的、商业化的啮齿类动物体内检测方法，特别是小鼠体内检测方法。为了解决这一缺陷，我们提出了一种创新的、自动化的、可操作的行为方法来评估啮齿类动物的疼痛，我们的长期目标是建立一个可快速、系统、有效地评估疼痛的操作模型系统。 中心假设是操作性测定可以提供一种敏感、可靠和临床相关的方法来评估感觉和伤害性处理。将研究一个具体目标：与用于研究疼痛的大鼠操作模型相比，评估热痛觉过敏和异常性疼痛的小鼠操作模型。我们将使用一种极其创新、自动化、高通量的方法来测试这一目标，以测试小鼠和大鼠的疼痛和镇痛作用。这包括使用奖励冲突策略来评估热痛。拟议研究的基本原理是，在大鼠和小鼠中使用这些操作测定来评估疼痛将为未来研究疼痛疾病的机制提供更好的方法。这项研究探索了一种评估疼痛动物模型的创新方法，并将通过提供使用啮齿动物的快速可靠的疼痛测试系统来显着推进新疗法的开发。待开发的产品和工艺是一种自动化、用户友好、独立于研究者、高通量的体内疼痛和镇痛测试系统。鉴于 Stoelting 公司在开发产品方面的专业知识和 Drs. 的配合，我们特别适合完成这项工作。纽伯特和考德尔的疼痛研究经历。这一系列研究意义重大，因为它探索了一种新颖的高通量方法来评估镇痛给药后的疼痛和疼痛缓解情况。如果这一系列研究取得成功，其结果将对新的止痛疗法的筛选和评估方式产生重大影响，最终大大缩短新药进入临床的时间。