Assay for rapid Poly(A) Tail length determination

快速测定 Poly(A) 尾长的分析方法

• 批准号: 7481545
• 负责人: LANCE P FORD
• 金额: $ 17.62万
• 依托单位: BIOO SCIENTIFIC CORPORATION
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2010-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7481545
• 关键词: Address; Adenosine; Area; Bioinformatics; Biological Assay; Biomedical Research; Cell Nucleus; Cells; Clear Cell; Development; Elements; Embryo; Ensure; Enzymes; Event; Fostering; Gene Expression; Generations; Genes; Goals; Human; Kinetics; Laboratories; Lead; Length; Ligation; Link; Measures; Mediating; Messenger RNA; Methods; Molecular; Molecular Biology; Nuclear; Numbers; Poly A; Poly(A) Tail; Polyadenylation; Polymerase Chain Reaction; Polynucleotide Adenylyltransferase; Population; Process; Property; Quality Control; RNA; RNA Ligase (ATP); Rate; Regulation; Research; Research Personnel; Role; Standards of Weights and Measures; Tail; Technology; Time; Transcript; Validation; base; improved; in vivo; insight; knock-down; poly U polymerase; success; tool

DESCRIPTION (provided by applicant): One of the major underlying factors for the significant broad impact of molecular biology on biomedical research is the generation of effective and highly reproducible assay kits to measure fundamental properties of gene expression. One area of molecular biology that is relatively understudied is the role of poly(A) tail length in the regulation of transcript fate and gene expression. Much of this is due to the absence of a highly reproducible means for the average biomedical research lab to assess this property of cellular mRNAs. Current assays have enjoyed varied success among laboratories and many standard molecular biology researchers simply shy away from assessing poly(A) tail length due to the lack of a readily available approach. The goal of this proposal is to address this need and develop and validate an efficient, highly reproducible approach to assess poly(A) tail length on a mRNA specific basis.

描述（由申请人提供）：分子生物学对生物医学研究产生重大广泛影响的主要潜在因素之一是产生有效且高度可重复的测定试剂盒，以测量基因表达的基本特性。一个研究相对不足的分子生物学领域是poly（A）尾长在转录物命运和基因表达调控中的作用。这在很大程度上是由于缺乏一个高度可重复的手段，平均生物医学研究实验室评估细胞mRNA的这种性质。目前的测定在实验室中取得了不同的成功，许多标准分子生物学研究人员由于缺乏现成的方法而根本不愿评估poly（A）尾长。本提案的目标是解决这一需求，并开发和验证一种有效的，高度可重复的方法来评估mRNA特异性基础上的poly（A）尾长。