Development of a selective biosensor for detecting organophosphate pesticide expo

描述（由申请人提供） 该SBIR项目是为了响应开发可现场部署或可穿戴的个人传感器的呼吁，以通过生物样品测试监测接触点对空气中化学品的暴露。该提案的广泛目标是开发一种极其灵敏和选择性的生物传感器装置，该装置能够检测和区分从疑似暴露于潜在有害水平的有机磷酸酯基农药的患者中采集的人血清样品中的蛋白质。这代表了生物标志物分析中的一种新方法，因为每种有机磷酸酯（OP）农药都会产生不同的蛋白质“指纹”结构，该结构可以被识别、与其他药剂及其缀合物区分开并被定量。使用ATERIS Technologies新型传感器薄膜聚合物技术，报告域将被定制为具有检测OP中毒蛋白的特定蛋白质识别分子。这将使得有可能开发一种廉价的，但高度快速，灵敏和准确的设备来分析暴露于OP农药，评估OP剂暴露的类型和程度，然后这些信息将指导必要的治疗干预。第一阶段SBIR项目的主要里程碑是首先分离出能够区分天然和OP中毒人类蛋白质的生物识别蛋白质，然后证明薄膜传感器元件的灵敏度和可靠性。在该项目的第II阶段，检测膜将被整合到读取器设备中，并将确定实际血清样品中OP修饰蛋白检测的再现性和准确性。所产生的数据将指导商业生物传感器装置的设计。此类器械的最终用户将是可能接触OP农药的现场人员和可能遇到疑似接触OP化学制剂的患者的临床实验室。

DESCRIPTION (provided by applicant) This SBIR project is in response to the call for the development of field-deployable or wearable personal sensors for monitoring point-of-contact exposures to airborne chemicals through biosample testing. The broad objective of this proposal is to develop an extremely sensitive and selective biosensor device capable of detecting and discriminating proteins in human serum samples taken from patients suspected of being exposed to potentially harmful levels of organophosphate-based pesticides. This represents a novel approach in biomarker analysis because each organophosphate (OP) pesticide results in a distinct protein "fingerprint" structure that can be identified, distinguished from other agents and their conjugates, and quantified. Using ATERIS Technologies novel sensor thin polymer film technology, reporter domains will be customized with specific protein-recognition molecules that detect the OP poisoned proteins. This will make it possible to develop an inexpensive, yet highly rapid, sensitive, and accurate device to analyze exposure to OP pesticides, assess the type and extent of OP agent exposure and then this information will guide the therapeutic intervention necessary. The major milestones in this Phase I SBIR project are first to isolate biorecognition proteins capable of discriminating between native and OP-poisoned human proteins, then show proof-of-principal for the sensitivity and reliability of the film sensor element. In the Phase II of this program, the detection films will be incorporated into a reader device and the reproducibility and accuracy of detection of OP-modified proteins in actual serum samples will be determined. The data generated will guide the design of the commercial biosensor device. The end user of such a device will be the field personnel likely to be exposed to OP pesticides and clinical laboratories likely to encounter patients that are suspected to have been exposed to OP chemical agents.