HISTOLOGY AND DIAGNOSTIC CORE

组织学和诊断核心

• 批准号: 7480358
• 负责人: VIVETTE D'AGATI
• 金额: $ 8.23万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7480358
• 关键词: Biopsy; Blinded; Classification; Diagnostic; Electron Microscopy; Electrons; Histology; Histopathology; Human; Immunofluorescence Immunologic; Immunohistochemistry; Kidney; Kidney Diseases; Light; Microscopic; Mus; Pattern; Process; Proteins; Purpose; Reagent; Renal Tissue; Score; Severity of illness; Signal Pathway; Techniques; Tissues; immunocytochemistry; interest; laser capture microdissection; novel; protein expression

The objective of Core B is to provide histopathology support, both technical and interpretative, for Projects 1 through 4. For Project 1, Core B will process murine tissues for light microscopic analysis, for laser capture microdissection and for electron microscopy. Scoring of murine tissues for disease severity will be performed blindly. For Project 2, core B will provide diagnostic expertise to classify human renal biopsies as HIVAN vs. non-HIVAN renal disease by integration of light microscopic, immunofluorescence and electron microscopic findings in blinded fashion. For Project 3, novel reagents to gene products of interest will be screened on normal and diseased human renal tissue and murine tissues by immunocytochemistry and immunoelectronmicroscopy. For Project 4, immunohistochemistry for proteins regulated by Nef signaling Pathways will be performed on murine and human renal tissues.

核心B的目的是为项目1到4提供组织病理学支持，无论是技术和解释性的。对于项目1而言，Core B将处理鼠组织以进行光显微镜分析，用于激光捕获显微解剖和电子显微镜。将盲目执行鼠组织评分疾病严重程度。对于项目2，Core B将通过以盲目的方式整合光显微镜，免疫荧光和电子显微镜发现，以将人类肾脏活检分类为Hivan vs.非Hivan肾脏疾病。对于项目3，通过免疫细胞化学和 免疫电子显微镜检查。对于项目4，将对由NEF信号通路调节的蛋白质进行免疫组织化学，将对鼠和人肾组织进行。