CONSTRUCTION OF A CHIMPANZEE GENETIC MAP FOR CHROMOSOME 20

黑猩猩 20 号染色体遗传图谱的构建

• 批准号: 7562583
• 负责人: DAVID SERRE
• 金额: $ 3.95万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562583
• 关键词: Address; Biological Process; Chromosome Mapping; Chromosomes; Chromosomes, Human, Pair 20; Computer Retrieval of Information on Scientific Projects Database; Custom; DNA Resequencing; Data; Disease; Europe; Facility Construction Funding Category; Female; Funding; Genes; Genetic Recombination; Genome; Genotype; Goals; Grant; Human; Institution; Linkage Disequilibrium; Location; Numbers; Pan Genus; Pan troglodytes; Physical Chromosome Mapping; Physical Map of the Human Genome; Pilot Projects; Primates; Process; Rate; Research; Research Personnel; Resolution; Resources; Scientist; Single Nucleotide Polymorphism; Source; United States National Institutes of Health; design; male; physical mapping; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recombination is a fundamental biological process about which we still know very little, especially in primates. Understanding the recombination process is of practical importance for the identification of disease genes in humans, as well as for evolutionary inference about humans and primates more generally. Large-scale recombination rates can be estimated by comparing physical and genetic maps, and while we now have a draft of the complete chimpanzee genome, very little is known about the genetic map for this species. In an effort to create a first genetic map for one chimpanzee chromosome and compare it to the physical map, we started a large scale collaborative program, involving scientists from the U.S. and Europe, and from the major chimpanzee holding facilities. We are addressing a number of topical questions, including: How do recombination rates vary along the chromosome and between males and females? How similar are rates between humans and chimpanzees? Our goal is to genotype over 80,000 single nucleotide polymorphisms across the genome using a custom-design Nimblegen array, allowing us to infer recombination locations with very high spatial resolution. As a pilot project, we have resequenced amplicons across the pseudo-autosomal regions in 32 unrelated chimpanzees, in order to infer fine-scale recombination rates from linkage disequilibrium data.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 重组是一个基本的生物过程，我们对其知之甚少，尤其是在灵长类动物中。了解重组过程对于识别人类疾病基因以及更广泛地推断人类和灵长类动物具有实际意义。大规模重组率可以通过比较物理图谱和遗传图谱来估计，虽然我们现在有了完整的黑猩猩基因组草图，但对该物种的遗传图谱知之甚少。 为了创建第一个黑猩猩染色体遗传图谱并将其与物理图谱进行比较，我们启动了一项大规模合作计划，涉及来自美国和欧洲以及主要黑猩猩收容设施的科学家。 我们正在解决许多热门问题，包括：沿着染色体以及男性和女性之间的重组率如何变化？人类和黑猩猩之间的比率有多相似？ 我们的目标是使用定制设计的 Nimblegen 阵列对基因组中超过 80,000 个单核苷酸多态性进行基因分型，使我们能够以非常高的空间分辨率推断重组位置。作为一个试点项目，我们对 32 只不相关黑猩猩的伪常染色体区域的扩增子进行了重新测序，以便从连锁不平衡数据推断精细重组率。