Tissue/Pathology Core

组织/病理学核心

• 批准号: 7727558
• 负责人: LAWRENCE S LAMB
• 金额: $ 23.15万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7727558
• 关键词: Address; Alabama; Aliquot; Biological; Biometry; Blood; Cataloging; Catalogs; Cells; Cervical; Cervix Uteri; Cervix carcinoma; Clinical; Clinical Trials; Collection; Colorado; Comprehensive Cancer Center; Confidentiality; Core Facility; Databases; Development; Diagnostic Services; Enrollment; Ensure; Epidemiologic Studies; Evaluation; Fees; Funding; Goals; Head and neck structure; Histologic; Human; Human Papillomavirus; Immunohistochemistry; Immunology; Immunology procedure; Immunophenotyping; In Situ Hybridization; Individual; Informed Consent; Laboratories; Lesion; Liquid substance; Location; Malignant neoplasm of cervix uteri; Mission; Normal tissue morphology; Online Systems; Pathologic; Pathology; Patient Care; Patients; Peripheral Blood Mononuclear Cell; Plasma; Practice Guidelines; Premalignant; Process; Quality Control; Reproduction spores; Research; Research Personnel; Resource Sharing; Route; Sampling; Secure; Services; Site; Specimen; Staging; Staining method; Stains; System; Testing; Tissue Microarray; Tissues; Translational Research; Universities; Whole Blood; abstracting; anticancer research; base; cost; data management; design; human tissue; laser capture microdissection; malignant breast neoplasm; neoplastic; programs; tissue preparation; web-enabled

This Tissue/Pathology Core provides well-characterized human biological specimens to researchers participating in this Cervical Cancer SPORE and other collaborative research efforts. In the first funding period this core collected over 78,000 individual aliquots from over 3524 patients enrolled by the SPORE in Cervical Cancer and distributed 6332 aliquots to date. This includes over 28,000 aliquots from 838 patients to date from a clinical trial and two epidemiologic studies of the JHU Breast Cancer and Head and Neck SPOREs at no additional cost. In addition to the growing need for sophisticated sample acquisition, our investigators depend on expert pathology support to ensure proper tissue preparation and characterization for selected studies. This Core provides such expert pathologic evaluation of specimens and technical support. Specifically, this core continues to: 1. Obtain informed consent and collect specimens from patients for translational research without compromise of patient care or confidentiality; 2. Collect cervical carcinoma and pre-malignant lesions, as well as normal tissue from patients, including those enrolled in clinical trials for the SPORE projects; 3. Collect blood, secretions and exfoliated cells (e.g. cervical scrapes) from patients, including those enrolled in clinical trials for the SPORE projects; 4. Process and store clinical specimens following SOPs to address the requirements of all SPORE investigators; 5. Input specimen information into central database system and track specimen distribution and transport; 6. Characterize tissue specimens with respect to site of origin, pathologic grading and staging, and proportion of neoplastic and stromal tissue; 7. Use well-defined mechanisms for prioritization of the distribution of requested specimens to investigators within and external to the Johns Hopkins SPORE; 8. Provide quality-controlled specimens in a timely fashion as inexpensively and efficiently as possible; 9. Route specimens for histologic and virologic analyses e.g. immunohistochemical staining, in situ hybridization, tissue microarraying, and HPV testing and typing, immunophenotyping, or laser-capture microdissection in fee-per-service Comprehensive Cancer Center core facilities; 10. Support the development and implementation of immunologic assays. The samples will be tracked using CaTissue, a CaBIG complaint database, web-enabled for access by our projects at UAB and UC. The activities of this Core will be integrated with those of the Administrative/Clinical Core A, the Biostatistics and Data Management Core B and the Immunology Core D to ensure that specimens and clinical information are appropriately catalogued and disseminated.

该组织/病理学核心为研究人员提供了具有良好特征的人类生物标本 参与本次子宫颈癌孢子等合作研究工作。在第一笔资金中 在此期间，该中心从超过3524名患者中收集了超过78,000份等量样本 宫颈癌，到目前为止分发了6332份等量。这包括来自838名患者的28,000多份等分 迄今来自JHU乳腺癌和头颈部的一项临床试验和两项流行病学研究 不收取额外费用的孢子。除了日益增长的复杂样品采集需求外，我们的 研究人员依靠专家的病理学支持来确保适当的组织准备和特征描述 用于精选的研究。该核心提供这样的专家病理评估的标本和技术 支持。具体地说，这一核心继续：1.获得知情同意并从患者那里收集样本 在不影响患者护理或保密的情况下进行转译研究；2.收集宫颈癌 和癌前病变，以及患者的正常组织，包括那些登记参加临床试验的患者 孢子突出；3.采集患者的血液、分泌物和脱落细胞(如宫颈刮痕)， 包括那些为孢子项目注册的临床试验；4.临床标本的加工和存储 遵循标准操作规程，满足所有孢子调查人员的要求；5.将标本信息输入 中央数据库系统和跟踪标本的分配和运输；6.组织标本的特征 关于起源部位、病理分级和分期，以及肿瘤和间质组织的比例； 7.使用明确界定的机制，确定向调查人员分发所要求的标本的优先顺序 约翰霍普金斯孢子内外；8.及时提供有质量控制的标本 尽可能低成本和高效率；9.为组织学和病毒学分析提供标本，例如 免疫组织化学染色、原位杂交、组织微阵列、HPV检测和分型， 按服务收费的综合癌症中心核心的免疫表型分析或激光捕获显微解剖 设施；10.支持开发和实施免疫学检测。样品将会是 使用CaTIssue进行跟踪，这是一个CaBIG投诉数据库，可供我们在UAB和UAB的项目访问 加州大学。这一核心的活动将与行政/临床核心A、 生物统计和数据管理核心B和免疫学核心D，以确保样本和 临床信息被适当地编目和传播。