CORE--Bioanalytical

核心--生物分析

• 批准号: 6893116
• 负责人: Bruce Alan Freeman
• 金额: $ 79.05万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6893116
• 关键词: analytical method; biological signal transduction; biomedical facility; biomedical resource; cardiovascular disorder diagnosis; cardiovascular disorder epidemiology; congestive heart failure; data quality /integrity; experimental designs; health science research support; immunologic techniques; inflammation; mass spectrometry; method development; microscopy; polymerase chain reaction; technology /technique development

The purpose of Bioanalytical services are to a) facilitate experimental aims being pursued in the context of both animal studies and clinical protocols, b) integrate key experimental goals c) provide consistent and responsive high quality analytical data that supports the hypothesis-testing and d) develop and apply new analytical methodologies that will meet the evolving needs and successes. The Bioanalytical faculty participants and their staff are established experts in inflammatory and vascular biology, cell signaling, proteomics, lipidomics and redox chemistry. They will bring to bear their individual expertise in collaborative and service functions designed to support the proposed goals of this SCCOR. Specific Bioanalytical objectives include a) providing sensitive and unambiguous measurement of indices of vascular and cardiac tissue inflammatory processes and their resolution, b) optimization and standardization of microscopic-, immunological-, mass spectrometric- and PCR-based techniques for the tissue localization, quantification and/or characterization of specific mRNAs and proteins, c) the application of sensitive and specific mass spectrometric approaches for the measurement of products of cardiovascular inflammatory signaling and their sequelae - specifically, oxidized fatty acids, and oxidized and nitrated proteins and d) the generation of new insight regarding inflammatory mechanisms and target molecules that contribute to CHF, that will in turn motivate future novel hypothesis-driven experimental and therapeutic approaches. In the integrated clinical and basic investigatory approach adopted by this SCCOR proposal, Bioanalytical will thus provide a consistent resource for measurement of key experimental endpoints serving as outcome variables related to progression and resolution of CHF. Use will ensure uniformity in a) blood, microdialysate and tissue sampling, b) biomaterial processing and c) sample storage between projects. We will also contribute to the design of quality control measures underlying procedures executed and the overall design and planning of experimental aims.

生物分析服务的目的是：a)促进正在进行的实验目标 在动物研究和临床方案的背景下，b)整合关键的实验目标，c)提供支持假设检验的一致和反应灵敏的高质量分析数据，以及d)开发和应用新的分析方法，以满足不断发展的需求和成功。生物分析学院的参与者和他们的工作人员都是炎症和血管生物学、细胞信号、蛋白质组学、 脂质组学和氧化还原化学。他们将运用他们在协作和服务职能方面的个人专长，以支持本SCCOR的拟议目标。具体的生物分析目标包括：a)提供敏感和明确的测量 这些研究包括：(A)血管和心脏组织炎症过程的指数及其分解；b)针对特定mRNA和蛋白质的组织定位、量化和/或表征的显微、免疫学、质谱学和聚合酶链式反应技术的优化和标准化；c)应用敏感和特异的质谱学方法测量心血管炎症信号产物及其后遗症--特别是氧化脂肪酸以及氧化和硝化蛋白质；d)对导致CHF的炎症机制和靶分子产生新的见解，这反过来将推动未来以假设为导向的新的实验和治疗方法。因此，在SCCOR建议采用的综合临床和基础研究方法中，Bioanalytic将为作为与CHF进展和解决相关的结果变量的关键实验终点的测量提供一致的资源。使用将确保a)血液、微透析液和组织采样的一致性，b) 生物材料加工和c)项目之间的样品存储。我们还将为 设计执行的基本程序和总体的质量控制措施 实验目标的设计和规划。