CORE--Bioanalytical

生物分析服务的目的是：a）促进在生物技术领域追求的实验目标， 在动物研究和临床方案的背景下，B）整合关键实验目标，c）提供支持假设检验的一致和响应性高质量分析数据，以及d）开发和应用新的分析方法，以满足不断变化的需求和成功。生物分析教师参与者及其工作人员是炎症和血管生物学，细胞信号传导，蛋白质组学， 脂质组学和氧化还原化学。他们将在协作和服务职能方面发挥各自的专长，以支持本SCCOR的拟议目标。具体的生物分析目标包括：a）提供灵敏和明确的测量， 血管和心脏组织炎症过程的指数及其分辨率，B）用于组织定位、定量和/或表征特定mRNA和蛋白质的基于显微镜、免疫学、质谱和PCR的技术的优化和标准化，c）应用灵敏和特异的质谱方法测量心血管炎症信号传导的产物及其后遗症-特别是氧化脂肪酸、氧化和硝化蛋白质，以及d）产生关于炎症机制和导致CHF的靶分子的新见解，这反过来将激发未来新的假设驱动的实验和治疗方法。因此，在本SCCOR提案采用的综合临床和基础阐明方法中，生物分析将为测量关键实验终点提供一致的资源，这些终点作为与CHF进展和消退相关的结局变量。 使用将确保a）血液、微透析液和组织采样的均匀性，B） 生物材料加工和c）项目之间的样本存储。我们还将为 设计所执行程序的质量控制措施， 实验目标的设计和规划。

The purpose of Bioanalytical services are to a) facilitate experimental aims being pursued in the context of both animal studies and clinical protocols, b) integrate key experimental goals c) provide consistent and responsive high quality analytical data that supports the hypothesis-testing and d) develop and apply new analytical methodologies that will meet the evolving needs and successes. The Bioanalytical faculty participants and their staff are established experts in inflammatory and vascular biology, cell signaling, proteomics, lipidomics and redox chemistry. They will bring to bear their individual expertise in collaborative and service functions designed to support the proposed goals of this SCCOR. Specific Bioanalytical objectives include a) providing sensitive and unambiguous measurement of indices of vascular and cardiac tissue inflammatory processes and their resolution, b) optimization and standardization of microscopic-, immunological-, mass spectrometric- and PCR-based techniques for the tissue localization, quantification and/or characterization of specific mRNAs and proteins, c) the application of sensitive and specific mass spectrometric approaches for the measurement of products of cardiovascular inflammatory signaling and their sequelae - specifically, oxidized fatty acids, and oxidized and nitrated proteins and d) the generation of new insight regarding inflammatory mechanisms and target molecules that contribute to CHF, that will in turn motivate future novel hypothesis-driven experimental and therapeutic approaches. In the integrated clinical and basic investigatory approach adopted by this SCCOR proposal, Bioanalytical will thus provide a consistent resource for measurement of key experimental endpoints serving as outcome variables related to progression and resolution of CHF. Use will ensure uniformity in a) blood, microdialysate and tissue sampling, b) biomaterial processing and c) sample storage between projects. We will also contribute to the design of quality control measures underlying procedures executed and the overall design and planning of experimental aims.