EMBRYONIC AND AGE-RELATED EXPRESSION OF AQP4, NHE2, AE2, KIR21 AND KVIQT1 IN TH
TH 中 AQP4、NHE2、AE2、KIR21 和 KVIQT1 的胚胎和年龄相关表达
基本信息
- 批准号:7725273
- 负责人:
- 金额:$ 8.4万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-07-01 至 2009-06-30
- 项目状态:已结题
- 来源:
- 关键词:AcidsAdultAgeAgingBiologyBirthCell membraneChloride ChannelsComputer Retrieval of Information on Scientific Projects DatabaseDevelopmentEmbryoEmbryonic DevelopmentFundingGastric AcidGastric Parietal CellsGastrinsGene ExpressionGene ProteinsGenesGenetically Engineered MouseGoalsGrantImpairmentInstitutionIon ExchangeMusNHE2PatternPotassium ChannelRegulationResearchResearch PersonnelResourcesSourceStagingStomachSystemThinkingTimeUnited States National Institutes of Healthage relatedaquaporin 4gastrointestinalprotein expressionwater channel
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Previous studies have shown that proper ion exchange and transport across the parietal cell membrane is essential for normal gastric acid secretion. For example, genetically engineered mice deficient for the Na+/H+ exchangers Nhe2 and Nhe4, the Cl-/HCO3 exchanger Ae2, and the K+ channel Kvlqt1 showed significant impairments in acid secretion. In addition, achlorhydric gastrin-deficient mice showed alterations in gastric expression of the water channel aquaporin-4 (Aqp4) and the chloride channel Clca3. The patterns of gastric expression of these genes during embryonic development or as mice age is unknown. Gastric acid secretion is thought to be initiated immediately before parturition; in addition, evidence suggests that there is an increase in gastric acid secretion as mice age. Understanding the expression patterns of Aqp4, Clca3, Nhe2, Nhe4, Ae2, Kir2.1, and Kvlqt1 both in development and in aging mice may yield clues as to the timing of initiation of acid secretion as well as the age-related increase in acid levels. The overall goal of this research is to analyze developmental and age-related expression of various channels and exchangers important for normal acid secretion, including Aqp4, Clca3, Nhe2, Nhe4, Ae2, Kir2.1, and Kvlqt1. The hypothesis of this study is that the gastric expression of these genes increases at later embryonic developmental stages and increases as adult mice age. Specific Aim 1 will focus on gene expression analysis and gastric immunolocalization of candidate molecules during embryonic development. Specific Aim 2 will analyze gene and protein expression patterns of these candidates in aging wild-type and gastrin-deficient mice. Determining the expression patterns of these genes in murine embryonic development as well as in aging mice will allow a more thorough understanding of mechanisms that that regulate the gastric acid secretory system. This research will have an impact on the field of gastrointestinal biology by further elucidating the developmental and age-related regulation of gastric acid secretion.
这个子项目是许多研究子项目中利用
资源由NIH/NCRR资助的中心拨款提供。子项目和
调查员(PI)可能从NIH的另一个来源获得了主要资金,
并因此可以在其他清晰的条目中表示。列出的机构是
该中心不一定是调查人员的机构。
以前的研究表明,适当的离子交换和跨壁细胞膜的运输对于正常的胃酸分泌是必不可少的。例如,缺乏Na+/H+交换器Nhe2和Nhe4、Cl2/HCO3交换器AE2和K+通道Kvlqt1的基因工程小鼠表现出显著的酸分泌障碍。此外,胃泌素缺乏的小鼠表现出胃水通道水通道4(Aqp4)和氯通道Clca3表达的改变。这些基因在胚胎发育期间或随着小鼠年龄增长而在胃中表达的模式尚不清楚。胃酸分泌被认为是在分娩前立即开始的;此外,有证据表明,随着老鼠年龄的增长,胃酸分泌也会增加。了解Aqp4、Clca3、Nhe2、Nhe4、AE2、Kir2.1和Kvlqt1在发育和衰老小鼠中的表达模式,可能会为酸分泌的开始时间以及与年龄相关的酸水平增加提供线索。本研究的总体目标是分析Aqp4、Clca3、Nhe2、Nhe4、AE2、Kir2.1和Kvlqt1等多种与正常酸分泌有关的通道和交换器的发育和年龄相关表达。这项研究的假设是,这些基因的胃部表达在胚胎发育后期增加,并随着成年小鼠年龄的增长而增加。具体目标1将侧重于胚胎发育过程中候选分子的基因表达分析和胃免疫定位。特定目标2将分析这些候选基因和蛋白质在衰老野生型和胃泌素缺陷小鼠中的表达模式。确定这些基因在小鼠胚胎发育和衰老小鼠中的表达模式,将有助于更彻底地了解调节胃酸分泌系统的机制。这项研究将进一步阐明胃酸分泌的发育和年龄相关的调节,从而对胃肠生物学领域产生影响。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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KAREN L HINKLE其他文献
KAREN L HINKLE的其他文献
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{{ truncateString('KAREN L HINKLE', 18)}}的其他基金
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TFM 处理的酿酒酵母中线粒体 DNA 损伤的研究
- 批准号:
8360425 - 财政年份:2011
- 资助金额:
$ 8.4万 - 项目类别:
INVESTIGATION OF LAMPRICIDE-INDUCED PROGRAMMED CELL DEATH IN S CEREVISIAE
杀藻剂诱导的酿酒酵母程序性细胞死亡的研究
- 批准号:
8168164 - 财政年份:2010
- 资助金额:
$ 8.4万 - 项目类别:
INVESTIGATION OF LAMPRICIDE-INDUCED PROGRAMMED CELL DEATH IN S CEREVISIAE
杀藻剂诱导的酿酒酵母程序性细胞死亡的研究
- 批准号:
7959878 - 财政年份:2009
- 资助金额:
$ 8.4万 - 项目类别:
EMBRYONIC AND AGE-RELATED EXPRESSION OF AQP4, NHE2, AE2, KIR21 AND KVIQT1 IN TH
TH 中 AQP4、NHE2、AE2、KIR21 和 KVIQT1 的胚胎和年龄相关表达
- 批准号:
7610057 - 财政年份:2007
- 资助金额:
$ 8.4万 - 项目类别:
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PACAP-1 受体启动子中调控序列的研究
- 批准号:
7381417 - 财政年份:2006
- 资助金额:
$ 8.4万 - 项目类别:
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人 PACAP1 受体基因表达的研究
- 批准号:
7170638 - 财政年份:2005
- 资助金额:
$ 8.4万 - 项目类别:
INVESTIGATION OF HUMAN PACAP1 RECEPTOR GENE EXPRESSION
人 PACAP1 受体基因表达的研究
- 批准号:
6981593 - 财政年份:2003
- 资助金额:
$ 8.4万 - 项目类别:
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