SOLUTE TRANSPORT IN THE SUBCHONDRAL BONE PLATE OF OSTEOARTHRITIC JOINTS

骨关节炎关节软骨下骨板中的溶质转运

• 批准号: 7720204
• 负责人: LIYUN WANG
• 金额: $ 22.68万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720204
• 关键词: Biological; Bone and Cartilage Funding; Bone plates; Cartilage; Communication; Computer Retrieval of Information on Scientific Projects Database; Fluorescence Recovery After Photobleaching; Foundations; Funding; Grant; In Situ; In Vitro; Institution; Interruption; Joints; Measurement; Mentors; Methods; Movement; Osteoarthrosis Deformans; Pathway interactions; Patients; Research; Research Personnel; Resources; Source; Time; United States National Institutes of Health; articular cartilage; base; bone; bone cell; in vivo; mathematical model; research study; solute

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent experiments demonstrate close interaction between cartilage and bone compartment in vitro and in vivo, where bone cells from OA patients can cause matrix breakdown of articular cartilage and interruption of contact with subchondral bone results in cartilage degeneration. However, the communication pathway that allows cartilage and bone to interact is not clear. Dr. Wang has recently developed a method based on Fluorescence Recovery After Photobleaching (FRAP) which allows direct measurement of solute movement in the bone in situ and in real time. Drs. Wang et al. propose to use this approach in combination with mathematical modeling to characterize the solute diffusivity of representative molecules through subchondral bone plate in normal and OA joints. This project will build upon the biological foundation of project #1. Dr. Duncan will serve as mentor.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 最近的实验表明，在体外和体内，软骨和骨室之间的密切相互作用，其中骨细胞从OA患者可以引起关节软骨的基质分解和中断与软骨下骨的接触导致软骨退化。然而，允许软骨和骨相互作用的通信途径尚不清楚。Wang博士最近开发了一种基于光漂白后荧光恢复（FRAP）的方法，该方法允许直接测量原位和真实的骨中的溶质运动。Drs. Wang等人建议使用该方法结合数学建模来表征代表性分子通过正常和OA关节中的软骨下骨板的溶质扩散率。该项目将建立在项目#1的生物基础上。邓肯博士将担任导师。