SOLUTE TRANSPORT IN THE SUBCHONDRAL BONE PLATE OF OSTEOARTHRITIC JOINTS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 最近的实验表明，软骨和骨室在体外和体内都有密切的相互作用，其中来自骨关节炎患者的骨细胞可导致关节软骨基质破坏，与软骨下骨接触中断，导致软骨退变。然而，使软骨和骨骼相互作用的通讯途径尚不清楚。王博士最近开发了一种基于光漂白后荧光恢复(FRAP)的方法，可以在原位和实时直接测量溶质在骨骼中的运动。王博士等人。建议使用该方法结合数学模型来表征具有代表性的分子在正常关节和骨关节炎关节软骨下骨板中的溶质扩散率。这个项目将建立在项目1的生物学基础上。邓肯博士将担任导师。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Recent experiments demonstrate close interaction between cartilage and bone compartment in vitro and in vivo, where bone cells from OA patients can cause matrix breakdown of articular cartilage and interruption of contact with subchondral bone results in cartilage degeneration. However, the communication pathway that allows cartilage and bone to interact is not clear. Dr. Wang has recently developed a method based on Fluorescence Recovery After Photobleaching (FRAP) which allows direct measurement of solute movement in the bone in situ and in real time. Drs. Wang et al. propose to use this approach in combination with mathematical modeling to characterize the solute diffusivity of representative molecules through subchondral bone plate in normal and OA joints. This project will build upon the biological foundation of project #1. Dr. Duncan will serve as mentor.