PERCHLORATE RESEARCH

高氯酸盐研究

• 批准号: 7719968
• 负责人: Frank Arthur von Hippel
• 金额: $ 3.22万
• 依托单位: UNIVERSITY OF ALASKA FAIRBANKS
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7719968
• 关键词: Analytical Chemistry; Computer Retrieval of Information on Scientific Projects Database; Control Groups; Doctor of Philosophy; Female; Fishes; Funding; Gasterosteidae; Goals; Grant; Hermaphroditism; Institution; Manuscripts; Methods; Oregon; Paper; Perchlorates; Publishing; Research; Research Personnel; Resources; Seasons; Source; Students; United States National Institutes of Health; Universities; male; reproductive; research study; survivorship

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. 1) Projects currently in progress My Ph.D. student Richard Bernhardt and I have been studying effects of perchlorate on stickleback fish for the past five years. So far, we have published an analytical chemistry methods paper with three colleagues1, and a paper with William Cresko of the University of Oregon demonstrating that perchlorate causes genetically-female stickleback to develop into functional hermaphrodites, and genetically-male fish to develop super large testes2. This manuscript, which was published in August, 2006, also demonstrates that perchlorate interferes with the expression of normal nuptial coloration and reduces survivorship. This study provides the first evidence that perchlorate causes masculinization and is capable of inducing functional hermaphroditism in a nonhermaphroditic vertebrate. Because of these findings, the study received significant media coverage3,4,5. 2) Objectives and goals for grant fiscal year starting May 2007 We plan to begin a two-year stickleback experiment in my lab this May with six treatment groups: control (background), 10 ppb, 50 ppb, 500 ppb, 3,000 ppb, and 30,000 ppb (30,000 corresponds to our experimental level of 30 ppm where we observed hermaphroditism in female stickleback). The stickleback reproductive season begins in May and ends in July, so we plan to have our experimental set up complete by late April. I have requested funds from INBRE to build the experimental set-up this winter.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 1）目前正在进行的项目 我的博士学位过去五年来，学生理查德·伯恩哈特和我一直在研究高氯酸盐对棘鱼的影响。 到目前为止，我们已经与三位同事发表了一篇分析化学方法论文1，并与俄勒冈大学的William Cresko发表了一篇论文，证明高氯酸盐导致基因上的雌性棘鱼发育成功能性雌雄同体，以及基因上的雄性鱼发育出超大睾丸2。 这份手稿于 2006 年 8 月发表，还表明高氯酸盐会干扰正常婚姻颜色的表达并降低存活率。 这项研究提供了第一个证据，证明高氯酸盐会导致雄性化，并能够在非雌雄同体脊椎动物中诱导功能性雌雄同体。 由于这些发现，该研究得到了媒体的广泛报道3,4,5。 2) 2007 年 5 月开始的财政年度的目的和目标 我们计划今年五月在我的实验室开始一项为期两年的刺鱼实验，有六个处理组：对照组（背景）、10 ppb、50 ppb、500 ppb、3,000 ppb 和 30,000 ppb（30,000 对应于我们观察到雌性刺鱼雌雄同体的实验水平 30 ppm）。 刺鱼繁殖季节从五月开始，七月结束，因此我们计划在四月下旬之前完成实验设置。 我已向 INBRE 申请资金，用于今年冬天建立实验装置。