PERCHLORATE RESEARCH

高氯酸盐研究

• 批准号: 7960093
• 负责人: Frank Arthur von Hippel
• 金额: $ 3.55万
• 依托单位: UNIVERSITY OF ALASKA FAIRBANKS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960093
• 关键词: Alaska; Analytical Chemistry; Computer Retrieval of Information on Scientific Projects Database; Control Groups; Disease; Doctor of Philosophy; Female; Fishes; Funding; Gasterosteidae; Goals; Grant; Hermaphroditism; Institution; Manuscripts; Methods; Oregon; Paper; Perchlorates; Publishing; Research; Research Personnel; Resources; Seasons; Source; Students; United States National Institutes of Health; Universities; environmental agent; male; reproductive; research study; survivorship

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. 1) Projects currently in progress My Ph.D. student Richard Bernhardt and I have been studying effects of perchlorate on stickleback fish for the past five years. So far, we have published an analytical chemistry methods paper with three colleagues1, and a paper with William Cresko of the University of Oregon demonstrating that perchlorate causes genetically-female stickleback to develop into functional hermaphrodites, and genetically-male fish to develop super large testes2. This manuscript, which was published in August, 2006, also demonstrates that perchlorate interferes with the expression of normal nuptial coloration and reduces survivorship. This study provides the first evidence that perchlorate causes masculinization and is capable of inducing functional hermaphroditism in a nonhermaphroditic vertebrate. Because of these findings, the study received significant media coverage3,4,5. 2) Objectives and goals for grant fiscal year starting May 2007 We plan to begin a two-year stickleback experiment in my lab this May with six treatment groups: control (background), 10 ppb, 50 ppb, 500 ppb, 3,000 ppb, and 30,000 ppb (30,000 corresponds to our experimental level of 30 ppm where we observed hermaphroditism in female stickleback). The stickleback reproductive season begins in May and ends in July, so we plan to have our experimental set up complete by late April. I have requested funds from INBRE to build the experimental set-up this winter.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 第一章 目前正在进行的项目 我的博士学生理查德·伯恩哈特和我在过去的五年里一直在研究高氯酸盐对棘鱼的影响。 到目前为止，我们已经与三位同事发表了一篇分析化学方法论文1，以及与俄勒冈州大学的William Cresko合作的一篇论文，证明高氯酸盐会导致遗传上雌性的棘鱼发育成功能性雌雄同体，并导致遗传上雄性的鱼发育出超大的睾丸2。 这份发表于2006年8月的手稿也表明，高氯酸盐干扰了正常的婚姻色彩的表达，降低了生存率。 这项研究提供了第一个证据表明，高氯酸盐导致雄性化，并能够诱导功能性两性畸形的非两性畸形的脊椎动物。 由于这些发现，这项研究得到了媒体的广泛报道3，4，5。 （二） 2007年5月开始的赠款财政年度的目标和目的 我们计划今年5月在我的实验室开始为期两年的刺鱼实验，有六个处理组：对照（背景），10 ppb，50 ppb，500 ppb，3,000 ppb和30,000 ppb（30,000对应于我们的实验水平30 ppm，我们在雌性刺鱼中观察到雌雄同体）。 刺鱼的繁殖季节从5月开始，7月结束，所以我们计划在4月下旬完成实验。 我已经向INBRE申请了资金，以便在今年冬天建立实验装置。