LOUISIANA COBRE: OBESITY & DIABETES RES: GENOMICS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 基因组学核心设施(GCF)提供DNA测序、DNA片段分析、DNA、蛋白质和RNA样本的定性和定量分析、定量PCR、微阵列RNA标记、斑点、杂交和扫描、机器人和生物信息学服务。提供个人和小组培训和咨询服务，用于序列分析、实时聚合酶链式反应和微阵列分析。 全球合作框架的实验室服务向任何研究人员提供。全球合作框架的目标是以低成本提供高质量的服务，以促进研究。基因组学核心设施服务的使用是按先到先得的原则安排的。使用仪器的等待时间很少超过一天，而且往往更短。 核心实验室面积约为900平方英尺。两个辅助实验室，每个约168平方英尺，用于微阵列杂交、扫描和微阵列斑点检测。实验室配备有仪器专用电路的应急电源，以及超纯水、气体、空气和真空电源。 该实验室配备有： 两台应用生物系统3130XL 16台毛细管遗传分析仪。这些仪器执行DNA测序和片段分析。它们将容纳96个井和384个井格式的模板。 配备了96孔、384孔和低密度阵列块的四个应用生物系统7900HT序列检测系统可用于定量PCR。 希望使用商业微阵列系统的研究人员可以使用应用生物系统1700微阵列系统和Illumina微阵列系统。用于蛋白质印迹AN的微阵列设备具有生产定制的寡核苷酸或cDNA微阵列的能力。幻灯片用配备了服务器臂的GeneMachine OmnigridMicroarrayer打印，并使用Perkin-Elmer ScanArray5000扫描。Spotfire软件和BioConductor R Script用于分析微阵列数据。 两个移液机器人，Becton Dickinson Biomek FX和Perkin Elmer Multiple Probe II，可用于机器人液体处理。这些仪器有助于高通量吸移384个井型板材。它们可以被编程用于大型移液项目。 使用启根旋转柱套件，启根巧立方机器人将处理多达12个样品，用于样品分离和提纯。 两台安捷伦2100生物分析仪用于蛋白质、DNA和RNA的分析和定量。 使用NanoDrop分光光度计对RNA和DNA进行定量。 奥德赛红外成像仪使用直接红外荧光检测分析。 对于大型DNA提取和纯化项目，使用的是启根AutoPure机器人。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Genomics Core Facility (GCF) provides DNA sequencing, DNA fragment analysis, qualitative and quantitative analysis of DNA, protein, and RNA samples, quantitative PCR, microarray RNA labeling, spotting, hybridization, and scanning, robotics, and bioinformatics services. Individual and small group training and consultation services are offered for sequence analysis, real-time PCR, and microarray analysis. The GCF laboratory services are available to any researcher. The goal of the GCF is to provide high quality service at low cost to facilitate research. Use of the Genomics Core Facility services are scheduled on a first come, first serve basis. The wait time for instrument use is rarely more than one day and is frequently shorter. The core laboratory is approximately 900 square feet. Two ancillary laboratories of approximately 168 square feet each house microarray hybridization, scanning, and microarray spotting. Laboratories are supplied with emergency power with dedicated circuits for instrumentation as well as ultrapure water, gas, air, and vacuum supplies. The laboratory is equipped with: Two Applied Biosystems 3130XL sixteen capillary genetic analyzers. These instruments perform DNA sequencing and fragment analysis. They will accommodate 96 well and 384 well format sample plates. Four Applied Biosystems 7900HT Sequence Detection Systems equipped with 96 well, 384 well, and low density array blocks are available for quantitative PCR. An Applied Biosystems 1700 microarray system and an Illumina microarray system are available for researchers who wish to use a commercial microarray system. The microarray facilityfor western blot an has the capability to produce custom oligonucleotide or cDNA microarrays. Slides are printed with a GeneMachine OmniGrid Microarrayer equipped with server arm and scanned using a Perkin-Elmer ScanArray 5000. Spotfire software and Bioconductor R Scripts are used for analysis of microarray data. Two pipetting robots, a Becton Dickinson Biomek FX and a Perkin Elmer MultiProbe II, are available for robotic liquid handling. These instruments facilitate high-throughput pipetting of 384 well format plates. They can be programmed for large pipetting projects. A Qiagen Qiacube robot will process up to twelve samples using Qiagen Spin column kits for sample isolation and purification. Two Agilent 2100 Bioanalyzers are used for protein, DNA, and RNA analysis and quantitation. A NanoDrop Spectrophotometer is used for RNA and DNA quantification. An Odyssey Infrared Imager uses direct infrared fluorescence detection analysis. For large DNA extraction and purification projects, a Qiagen AutoPure robot is used.