LOUISIANA COBRE: OBESITY & DIABETES RES: GENOMICS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 彭宁顿生物医学研究中心基因组核心设施(PBRC-GCF)的目标是为路易斯安那州的路易斯安那州立大学社区和周围机构提供高通量DNA测序、实时聚合酶链式反应和微阵列能力。项目研究中心-全球合作框架网站(http://gcf.pbrc.edu/)，)已被项目研究中心研究人员和该区域几个机构的研究人员广泛用于自动提交样品、检索数据和开具账单。我们配备了一台ABI 3700(96毛细管)和两台ABI 3100(16毛细管)遗传分析仪(S)，有能力每天对超过一百万个碱基进行测序。支持使用来自ABI(1700平台)的预印幻灯片进行微阵列分析。生物信息学支持也将提供给研究人员，以遵循MIAME概述的指导方针，MIAME是一项标准化微阵列出版的国际努力。PBRC-GCF配备了机器人(Biomek FX机器人工作站，Beckman-Coulter)来支持高通量DNA测序和微阵列技术，以及两个实时PCR序列平台(ABI 7700和ABI 7900)，用于验证微阵列结果和定量RT-PCR。PBRC-GCF由莱斯·科扎克博士指导，苏珊·纽曼管理，盖尔·基尔罗伊和戴安娜·霍姆斯协助。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of the Pennington Biomedical Research Centers Genomics Core Facility (PBRC-GCF) is to provide high-throughput DNA sequencing, real time PCR and microarray capabilities for the LSU community and surrounding institutions in the State of Louisiana. The PBRC-GCF website (http://gcf.pbrc.edu/), has been used extensively for automated sample submission, data retrieval and billing by PBRC researchers and researchers at several institutions in the region. We are equipped with one ABI 3700 (96-capillary) and two ABI 3100s (16-capillary) genetic analyzer(s) and have the capability to sequence more than ¿ million basepairs a day. Support for microarray analysis using preprinted slides from ABI (1700 platform) is provided. Bioinformatics support will also be available to investigators to follow the guidelines outlined by MIAME, an international effort to standardize publication of microarray. The PBRC-GCF is equipped with robotics (Biomek FX robotic workstation, Beckman-Coulter) to support the high-throughput DNA sequencing and microarray technologies, and, two real-time PCR sequence platforms (ABI 7700; ABI 7900) for verification of microarray results and quantitative RT-PCR. The PBRC-GCF is directed by Dr. Les Kozak, managed by Susan Newman and assisted by Gail Kilroy and Diana Holmes.