LOUISIANA COBRE: OBESITY & DIABETES RES: GENOMICS CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。彭宁顿生物医学研究中心基因组核心设施（PBRC-GCF）的目标。为LSI社区和路易斯安那州的周边机构提供高通量DNA测序，实时PCR和微阵列功能。中国银监会-绿色气候基金会网站（http://gcf.pbrc.edii/）。该系统于2001年春季公布，已被该区域的几个机构广泛用于自动提交样品、检索数据和计费。该网站正在不断修改，以满足彭宁顿研究人员和周围科学界的需求。我们配备了一台ab13700（96-毛细管）和两台ABI 3100s（16-毛细管）基因分析仪，每天能够测序超过100万个碱基对。PBRC- gcf微阵列设备生产高密度微阵列，这些微阵列来自小鼠（操纵子13,442和最近更新的16,463个寡核苷酸文库）和人类（Compugen. 18,861寡核苷酸文库）以及PBRC科学家生成的几个cDNA文库。载玻片由配备服务器臂的GeneMachine OmniGrid微阵列打印，并使用GS1 Lumonics ScanArray 5000进行扫描。生物信息学支持也将提供给研究人员，我们目前正在开发微阵列数据和信息数据库，遵循MIAMK概述的指导方针。MIAMK是一项国际努力，旨在标准化微阵列数据，以提供有关微阵列实验的最少信息。PBRC-GCF配备机器人（Biomek FX机器人工作站，Beckman-Coulter），以支持高通量DNA测序和微阵列技术，并配备两个实时PCR序列检测系统（ABI 7700; ABI 7900），用于验证微阵列结果和定量RT-PCR。PBRC-GCF由Leslie P. Kozak博士和Robert A. KoZa博士领导，Susan Newman在Rebecca McCabe的协助下管理。除了服务之外，核心还将开发新的方法，以加强PBRC科学家的研究活动。最初的开发将是一个CpG岛微阵列，用于研究与成人疾病相关的表观遗传变化的研究人员。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of the Pennington Biomedical Research Centers Genomlcs Core Facility (PBRC-GCF) ).s to provide high-throughput DNA sequencing, real time PCR and microarray capabilities for the LSI) community and surrounding institutions in the State of Louisiana. The PBRC-GCF website (http://gcf.pbrc.edii/). made public during the spring of 2001, has been used extensively for automated sample submission, data retrieval and billing by several institutions in the region. The website is continually being modified 1:0 suit the needs of research staff at Pennington and the surrounding scientific community. We are equipped with one AB1 3700 (96-capillary) and two ABI 3100s (16-capillary) genetic analyzer(s) and have the capability to sequence more than Vz million basepairs a day. The PBRC-GCF micro array facility produces high-density micro arrays from mouse (Operon, 13,442 and recently updated 16,463 oligonucleotide libraries) and human (Compugen. 18,861 oligonucleotide library) and several cDNA libraries generated by scientists at PBRC. Slides are printed with a GeneMachine OmniGrid Microarrayer equipped with server arm and scanned using a GS1 Lumonics ScanArray 5000. Bioinformatics support will also be available to investigators and we are presently developing databases for microarray data and information following the guidelines outlined by MIAMK, an international effort to standardize microarray data to provide minimum information about a microarray experiment. The PBRC-GCF is equipped with robotics (Biomek FX robotic workstation, Beckman-Coulter) to support the high-throughput DNA sequencing and microarray technologies, and, two real-time PCR sequence detection systems (ABI 7700; ABI 7900) for verification of microarray results and quantitative RT-PCR. The PBRC-GCF is directed by Dr. Leslie P. Kozak and Dr. Robert A. KoZa arid managed by Susan Newman with assistance from Rebecca McCabe. In addition to services, the Core will develop new methodologies that will enhance the research activities of PBRC scientists. The initial development will be a CpG island microarray for the benefit of researchers working on cpigenetic changes in relation to adult disease.