MOLECULAR BIOLOGY AND BIOCHEMISTRY SUPPORT CORE

分子生物学和生物化学支持核心

• 批准号: 7720922
• 负责人: ROMAN R. GANTA
• 金额: $ 70.12万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720922
• 关键词: Academic Medical Centers; Biochemistry; Bioinformatics; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; DNA; Data; Funding; Gene Chips; Gene Expression; Genes; Grant; Individual; Institution; Kansas; Mentors; Molecular Biology; Nucleotides; Outsourcing; Pattern; Polymerase Chain Reaction; Preparation; Process; Processed Genes; Proteins; RNA; Research; Research Personnel; Resources; Sampling; Services; Source; Technology; Time; Training; Transcript; United States National Institutes of Health; Universities; Validation; cell type

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core C, the Molecular Biology and Biochemistry Support facility, provides the interface, expertise, training and mentoring in gene array analysis necessary to advance COBRE projects. Gene array technology provides a highly efficient means to screen individual cell types for gene expression patterns and changes. Gene microarray processing and bioinformatics services are available through the auspices of a K-INBRE NIH grant, thereby allowing us to focus Core support on sample preparation (analyses of quality and quantity), funding of gene microarray processing by other facilities at KSU and KUMC and of post-gene microarray validation (qRT-PCR). The Core provides gene array support by outsourcing to other facilities (at Kansas State University and Kansas University Medical Center) investigator RNA samples for gene chip preparation and hybridization; in addition, bioinformatics support to process the gene array data is provided by the COBRE Core Manager and by the service referenced above. The Core provides support through: 1) three, real-time polymerase chain reaction (PCR) thermocyclers for quantitative assessment of gene transcript expression, 2) an Agilent BioAnalyzer for determining the quality of RNA samples obtained for gene array analysis, 3) a Nanodrop spectrophotometer and spectrofluorometer for determining the quantity of nucleotide and protein samples, and 4) a DNA sequencer for verifying PCR product identity. The real-time PCR is needed to verify gene array results or analyze specific gene expression changes in more detail than is possible with the gene array.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 Core C是分子生物学和生物化学支持设施，在基因阵列分析中提供了界面，专业知识，培训和指导，以推进毛病项目。基因阵列技术提供了一种高效的手段，用于筛选单个细胞类型，以实现基因表达模式和变化。基因微阵列处理和生物信息学服务可通过K-Inbre NIH赠款的主持人获得，从而使我们能够将核心支持集中在样本制备上（质量和数量分析），基因微阵列在KSU和KUMC和KUMC和KUMC以及GESENE MIDRARARAY POESE MICRARRARAY VAINSITION（QRT-PRT）（QRT-PCR）的资金。核心通过将基因阵列支持提供给其他设施（堪萨斯州立大学和堪萨斯大学医学中心）的研究者RNA样品进行基因芯片准备和杂交；此外，生物信息学支持以处理基因数组数据由Cobre Core Manager和上述服务提供。核心通过以下方面提供支持：1）三个实时聚合酶链反应（PCR）热循环器，用于对基因转录表达的定量评估，2）一种用于确定基因阵列分析获得的RNA样品质量的敏捷生物分析仪，3）用于确定纳米光谱仪和光谱仪的量子和光谱元素的量子，并确定核酸元素的量子元素，并确定核酸元素的源于核酸元素的元素。验证PCR产品标识。需要实时PCR来验证基因阵列结果或分析比基因阵列可能更详细的特定基因表达变化。