MOLECULAR BIOLOGY AND BIOCHEMISTRY SUPPORT CORE

分子生物学和生物化学支持核心

• 批准号: 8360334
• 负责人: ROMAN R. GANTA
• 金额: $ 25.06万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360334
• 关键词: Biochemistry; Centers of Research Excellence; DNA Sequence; Data Analyses; Disease; Epithelial; Equipment; Equipment and Supplies; Funding; Gene Expression; Genes; Grant; Health; Investigation; Liquid substance; Mentors; Molecular Biology; National Center for Research Resources; Principal Investigator; Process; Protein Analysis; Reaction; Research; Research Infrastructure; Resources; Reverse Transcriptase Polymerase Chain Reaction; Robot; Robotics; Running; Sampling; Source; System; Time; Training; Transcript; United States National Institutes of Health; Western Blotting; cost; instrument; instrumentation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Core C, the Molecular Biology and Biochemistry Support Core, provides instrumentation, expertise, training and mentoring in gene expression analysis. COBRE and KSU projects use Core resources to make comparisons in gene expression under various experimental conditions. The Core provides gene array processing and data analysis, quantitative RT-PCR transcript analysis with robotic setup, DNA sequencing for verifying PCR product identity, Western blot protein analysis, and purchases common-use equipment and supplies for molecular biology and biochemistry investigations. This core has evaluated usage of equipment and as a result has traded in the obsolete Bio-Rad thermocyclers and replaced them with two StepOnePlus Real-time PCR Systems from Applied Biosystems. These instruments efficiently run PCR reactions on samples in 96-well plates, in conjunction with our liquid-handling robot that loads the plates.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 核心C，分子生物学和生物化学支持核心，提供基因表达分析的仪器，专业知识，培训和指导。 COBRE和KSU项目使用核心资源在各种实验条件下对基因表达进行比较。 核心提供基因阵列处理和数据分析，定量RT-PCR转录分析与机器人设置，DNA测序验证PCR产物的身份，蛋白质印迹分析，并购买常用的设备和用品的分子生物学和生物化学研究。 该核心评估了设备的使用情况，因此将过时的Bio-Rad热循环仪换成了Applied Biosystems的两个StepOnePlus实时PCR系统。 这些仪器与我们装载平板的液体处理机器人配合使用，可在96孔板中的样品上高效运行PCR反应。