MOLECULAR BIOLOGY SUPPORT CORE

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core C, the Molecular Biology Support facility, provides the interface, expertise, training and mentoring in gene array analysis necessary to advance COBRE projects. Gene array technology provides a highly efficient means to screen individual cell types for gene expression patterns and changes. Gene microarray processing and bioinformatics services are available through the auspices of a K-INBRE NIH grant, thereby allowing us to focus Core support on sample preparation (analyses of quality and quantity), funding of gene microarray processing by other facilities at KSU and KUMC and of post-gene microarray validation (qRT-PCR). The Core provides gene array support by outsourcing to other facilities (at Kansas State University and Kansas University Medical Center) investigator RNA samples for gene chip preparation and hybridization; in addition, bioinformatics support to process the gene array data is provided by the COBRE Core Manager and by the service referenced above. The Core provides support through: 1) two, real-time polymerase chain reaction (PCR) thermocyclers for quantitative assessment of gene transcript expression, 2) an Agilent BioAnalyzer for determining the quality of RNA samples obtained for gene array analysis, 3) a Nanodrop spectrophotometer and spectrofluorometer for determining the quantity of nucleotide and protein samples, and 4) a DNA sequencer for verifying PCR product identity. The real-time PCR is needed to verify gene array results or analyze specific gene expression changes in more detail than is possible with the gene array.