MOLECULAR BIOLOGY SUPPORT CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 核心C是分子生物学支持设施，提供推进Cobre项目所需的基因阵列分析方面的接口、专业知识、培训和指导。基因阵列技术提供了一种高效的手段来筛选单个细胞类型的基因表达模式和变化。基因微阵列处理和生物信息学服务是通过K-INBRE NIH赠款提供的，从而使我们能够将核心支持集中在样品准备(质量和数量分析)、由KSU和KUMC的其他设施为基因微阵列处理提供资金以及基因后微阵列验证(qRT-PCR)上。核心通过外包其他设施(堪萨斯州立大学和堪萨斯大学医学中心)研究人员RNA样本进行基因芯片准备和杂交来提供基因阵列支持；此外，处理基因阵列数据的生物信息学支持由Cobre核心经理和上述服务提供。该中心通过以下方式提供支持：1)两台实时聚合酶链式反应(PCR)热循环仪，用于定量评估基因转录表达；2)一台安捷伦生物分析仪，用于确定为进行基因阵列分析而获得的RNA样本的质量；3)一台纳米滴分光光度计和荧光分光光度计，用于确定核苷酸和蛋白质样本的数量；以及4)一台DNA测序仪，用于验证PCR产品的特性。需要实时聚合酶链式反应来验证基因阵列的结果或分析特定的基因表达变化，比基因阵列更详细。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core C, the Molecular Biology Support facility, provides the interface, expertise, training and mentoring in gene array analysis necessary to advance COBRE projects. Gene array technology provides a highly efficient means to screen individual cell types for gene expression patterns and changes. Gene microarray processing and bioinformatics services are available through the auspices of a K-INBRE NIH grant, thereby allowing us to focus Core support on sample preparation (analyses of quality and quantity), funding of gene microarray processing by other facilities at KSU and KUMC and of post-gene microarray validation (qRT-PCR). The Core provides gene array support by outsourcing to other facilities (at Kansas State University and Kansas University Medical Center) investigator RNA samples for gene chip preparation and hybridization; in addition, bioinformatics support to process the gene array data is provided by the COBRE Core Manager and by the service referenced above. The Core provides support through: 1) two, real-time polymerase chain reaction (PCR) thermocyclers for quantitative assessment of gene transcript expression, 2) an Agilent BioAnalyzer for determining the quality of RNA samples obtained for gene array analysis, 3) a Nanodrop spectrophotometer and spectrofluorometer for determining the quantity of nucleotide and protein samples, and 4) a DNA sequencer for verifying PCR product identity. The real-time PCR is needed to verify gene array results or analyze specific gene expression changes in more detail than is possible with the gene array.