TB STRUCTURAL GENOMICS CONSORTIUM

结核病结构基因组学联盟

• 批准号: 7721991
• 负责人: THOMAS C ALBER
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721991
• 关键词: Attenuated; Binding; Biochemical; Communicable Diseases; Computer Retrieval of Information on Scientific Projects Database; Crystallization; Extracellular Domain; Funding; Grant; Infection; Institution; Integral Membrane Protein; Ligands; Mediating; Mycobacterium tuberculosis; Population; Research; Research Personnel; Resolution; Resources; Source; Tuberculosis; United States National Institutes of Health; Virulence; Virulence Factors; insight; killings; pathogenic bacteria; protein structure; research study; structural genomics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The causative agent of tuberculosis is Mycobacterium tuberculosis (Mtb). This pathogenic bacterium infects one third of the world's population and kills over two million people annually, more than any other infectious disease. The TB Structural Genomics Consortium is aimed at determining the crystal structures of the proteins that mediate infection and persistence. Our group has crystallized the extra-cellular domain of an integral membrane protein which has been shown to be a virulence factor for TB. The crystals are highly diffracting (1.2 ¿ resolution). We have preliminary biochemical evidence as to the identity of the ligand for this extracellular domain and will be attempting both co-crystallisation and soaking experiments to determine the binding mechanism. Identification of this ligand and its binding mechanism is expected to give new insights into TB virulence and may provide a new target for attenuating TB infection.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 结核病的病原体是结核分枝杆菌（Mtb）。 这种致病细菌感染了世界三分之一的人口，每年导致超过 200 万人死亡，比任何其他传染病都要多。结核病结构基因组学联盟旨在确定介导感染和持久性的蛋白质的晶体结构。 我们的小组已经结晶了一种整合膜蛋白的胞外结构域，该蛋白已被证明是结核病的毒力因子。 晶体具有高度衍射性（1.2 ¿ 分辨率）。 我们有关于该胞外结构域配体身份的初步生化证据，并将尝试共结晶和浸泡实验来确定结合机制。 该配体及其结合机制的鉴定有望为结核病毒力提供新的见解，并可能为减轻结核病感染提供新的靶点。