IDENTIFYING THE METAL SITE ENVIRONMENTS IN P19 WITH EXAFS

使用 EXAFS 识别 P19 中的金属现场环境

• 批准号: 7722136
• 负责人: Tzanko Doukov
• 金额: $ 0.11万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722136
• 关键词: Bacteria; Binding; Campylobacter jejuni; Computer Retrieval of Information on Scientific Projects Database; Copper; Disruption; Environment; Funding; Genes; Genomics; Grant; Human; Institution; Iron; Metals; Oxygen; Periplasmic Proteins; Production; Proteins; Research; Research Personnel; Resources; Site; Source; Swimming; Thinking; United States National Institutes of Health; Water; foodborne; magnetic field; pathogen; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. P19 is a small (17500 Da) periplasmic protein isolated from the bacterium Campylobacter jejuni, a common food borne human pathogen. In C. jejuni, P19 expression is both iron and oxygen regulated (Palyada et al 2004). ChpA (copper handling protein) is a P19 orthologue characterized in a strain of magnetotactic bacteria. These unusual bacteria contain an intracellular magnetosome formed from magnets aligned as an arrow (Blakemore, 1975; Bazylinski & Frankel, 2004). This magnetosome is thought to sense the earth magnetic field enabling the bacteria to swim to the microaerobic layer of the water pool (pond, reservoir). Disruption of the gene for ChpA has shown that this protein is required for magnetosome production (Dubbels et al, 2004). ChpA as isolated from magnetotatic bacteria was shown to be a homodimer that binds copper. Inspection of the genomic context of both ChpA and P19 suggests that these orthologues are likely involved in iron uptake.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 P19是从空肠弯曲杆菌（一种常见的食源性人类病原体）分离的小（17500 Da）周质蛋白。In C.在空肠中，P19表达受铁和氧调节（Palyada等2004）。ChpA（铜处理蛋白）是一种P19直向同源物，其特征在于趋磁细菌菌株。这些不寻常的细菌含有一个细胞内的磁小体，它是由排列成箭头的磁铁形成的（Blakemore，1975; Bazylinski & Frankel，2004）。这种磁小体被认为可以感应地球磁场，使细菌能够游到水池（池塘，水库）的微需氧层。ChpA基因的破坏表明，该蛋白质是磁小体产生所必需的（Dubbels等人，2004）。从趋磁细菌中分离的ChpA显示为结合铜的同二聚体。对ChpA和P19的基因组背景的检查表明，这些直系同源物可能参与铁摄取。