DISSECTING AN ANTIBIOTIC FACTORY: OBTAINING THE STRUCTURE OF A POLYKETIDE SYNTHA

剖析抗生素工厂：获得聚酮合成物的结构

• 批准号: 7722011
• 负责人: Adrian Tristan Keatinge-Clay
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722011
• 关键词: Antibiotics; Antineoplastic Agents; Architecture; Carbon; Catalysis; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Funding; Grant; Immunosuppressive Agents; Institution; Pharmacologic Substance; Research; Research Personnel; Resolution; Resources; Source; Specificity; Structure; United States National Institutes of Health; polyketide synthase

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Polyketide synthases synthesize a wide variety of pharmaceuticals including anti-cancer agents, immunosuppressants, and antibiotics. The synthases are multienzyme assembly line that can be broken down into clusters of enzymes called modules that catalyze the addition of small carbon building blocks growing carbon chains. We are trying to understand the architecture of a module and the details of catalysis at atomic resolution. Only then will we be able to hypothesize how catalysis is achieved, including the mechanisms enabling specificity and stereoselectivity.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 聚酮酶合成多种药物，包括抗癌剂、免疫抑制剂和抗生素。这些酶是多酶组装线，可以分解成称为模块的酶簇，这些模块催化添加小碳构建块以生长碳链。我们正试图了解一个模块的架构和原子分辨率的催化细节。只有这样，我们才能够假设催化是如何实现的，包括实现特异性和立体选择性的机制。