GRAZING INCIDENCE SAXS ON SUBSTRATE SUPPORTED LIPID MEMBRANES
GRAZING INCIDENCE SAXS ON SUBSTRATE SUPPORTED LIPID MEMBRANES
批准号:
7722047
负责人:
THOMAS F WEISS
金额:
$0.33万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2008
资助国家:
美国
项目状态:
已结题
起止时间:
2008-03-01 至 2009-02-28
关键词:
AirComputer Retrieval of Information on Scientific Projects DatabaseFundingGramicidinGrantHumidityHydration statusIncidenceInstitutionIon ChannelLateralLipidsMediatingMembraneMembrane LipidsPeptidesPhospholipidsProteinsRelative (related person)ResearchResearch PersonnelResourcesSamplingSeriesSourceStructureSurfaceTemperatureThickUnited States National Institutes of HealthWaterliquid crystalprotein distributionresearch studyself assembly
中文摘要
这个子项目是众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Highly aligned stacks of lipid membranes can be prepared by self assembly on flat smooth surfaces forming a smectic-C liquid crystal structure with water intercalated between the bilayers formed by the lipid molecules. Such samples of aligned membranes have been extensively used for diffraction experiments concentrating on the structure of the membrane along the bilayer normal. In studying lipid-protein interactions it is also highly informative to look at the lateral distribution of the proteins in the membrane plane. We started a series of experiments on aligned phospholipid membranes of different thickness containing the dimeric ion channel Gramicidin. Using the newly developed sample chamber with humidity and temperature control, we investigated the change in the nearest neighbor distance between the peptides inside the membrane as a function of thickness and hydration state of the membrane. We find that the average peptide-to-peptide distance increases with increasing thickness of the bilayer. This effect is attributed to the increased repulsive interaction of the peptides due to the hydrophobic mismatch between the peptide and the lipid. In addition to the membrane mediated interaction between the peptides within the same membrane plane, we found that below a relative air humidity of 85% the peptide in neighboring membranes start to interact with each other leading to a partial ordering of of the peptides across the membrane stack.
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THE STRUCTURE OF INTERLEUKIN-33 AND ITS INTERACTION WITH THE ST2 AND IL-1RACP RE
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批准号:8362106
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项目类别:
-
资助金额:$0.14万
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财政年份:2011
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负责人:THOMAS F WEISS
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依托单位:
MEMBRANE MEDIATED PROTEIN INTERACTION
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批准号:8362133
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项目类别:
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资助金额:$0.14万
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财政年份:2011
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负责人:THOMAS F WEISS
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依托单位:
IMPROVEMENT OF X-RAY BEAM POSITION STABILITY FOR THE BL4-2 SAXS FACILITY
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批准号:8362095
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项目类别:
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资助金额:$1.37万
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财政年份:2011
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负责人:THOMAS F WEISS
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依托单位:
IMPROVEMENT OF X-RAY BEAM POSITION STABILITY FOR THE BL4-2 SAXS FACILITY
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批准号:8169998
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项目类别:
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资助金额:$8.48万
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财政年份:2010
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负责人:THOMAS F WEISS
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依托单位:
MEMBRANE MEDIATED PROTEIN INTERACTION
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批准号:8170062
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项目类别:
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资助金额:$0.44万
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财政年份:2010
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负责人:THOMAS F WEISS
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依托单位:
EVALUATION OF PARASITIC SCATTERING FROM WINDOW MATERIALS IN SAXS MEASUREMENTS
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批准号:8170077
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项目类别:
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资助金额:$3.05万
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财政年份:2010
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负责人:THOMAS F WEISS
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依托单位:
USER ORIENTATION, TRAINING AND SUPPORT FOR BIO-SAXS/D DATA ACQUISITION AND DATA
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批准号:8169962
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项目类别:
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资助金额:$3.39万
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财政年份:2010
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负责人:THOMAS F WEISS
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依托单位:
THE STRUCTURE OF INTERLEUKIN-33 AND ITS INTERACTION WITH THE ST2 AND IL-1RACP RE
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批准号:8170012
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项目类别:
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资助金额:$0.44万
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财政年份:2010
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负责人:THOMAS F WEISS
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依托单位:
MEMBRANE MEDIATED PROTEIN INTERACTION
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批准号:7954386
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项目类别:
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资助金额:$0.04万
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财政年份:2009
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负责人:THOMAS F WEISS
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依托单位:
IMPROVEMENT OF X-RAY BEAM POSITION STABILITY FOR BL4-2
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批准号:7954286
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项目类别:
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资助金额:$0.62万
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财政年份:2009
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负责人:THOMAS F WEISS
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依托单位:
USER ORIENTATION, TRAINING AND SUPPORT FOR BIO-SAXS/D DATA ACQUISITION AND DATA
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批准号:7954237
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项目类别:
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资助金额:$1.04万
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财政年份:2009
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负责人:THOMAS F WEISS
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依托单位:
A NEW BEAM INTENSITY MONITOR, BASED ON AVALANCHE PHOTO DIODE
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批准号:7721945
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项目类别:
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资助金额:$0.38万
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财政年份:2008
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负责人:THOMAS F WEISS
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依托单位:
ANOMALOUS X-RAY SCATTERING ON LIPID BICELLES
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批准号:7721813
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项目类别:
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资助金额:$0.34万
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财政年份:2008
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负责人:THOMAS F WEISS
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依托单位:
LIPID DIFFRACTION ON SUBSTRATE SUPPORTED LIPID MEMBRANE SYSTEMS
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批准号:7722068
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项目类别:
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资助金额:$0.57万
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财政年份:2008
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负责人:THOMAS F WEISS
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依托单位:
A NEW SAMPLE CHAMBER WITH TEMPERATURE AND HUMIDITY CONTROL FOR LIPID AND FIBER S
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批准号:7721944
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项目类别:
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资助金额:$0.57万
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财政年份:2008
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负责人:THOMAS F WEISS
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依托单位:
GRAZING INCIDENCE SAXS ON SUBSTRATE SUPPORTED LIPID MEMBRANES
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批准号:7598022
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项目类别:
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资助金额:$0.22万
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财政年份:2007
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负责人:THOMAS F WEISS
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依托单位:
A NEW SAMPLE CHAMBER WITH TEMPERATURE AND HUMIDITY CONTROL FOR LIPID AND FIBER S
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批准号:7598186
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项目类别:
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资助金额:$0.79万
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财政年份:2007
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负责人:THOMAS F WEISS
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依托单位:
X-RAY CONTRAST VARIATION IN LIPID SUSPENSIONS USING SUCROSE SOLUTION
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批准号:7598021
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项目类别:
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资助金额:$0.22万
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财政年份:2007
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负责人:THOMAS F WEISS
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依托单位:
EFFECTS OF LIPID COMPOSITION ON SIZE AND POLYDISPERSITY OF SELF-ASSEMBLED UNILAM
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批准号:7598101
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项目类别:
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资助金额:$0.24万
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财政年份:2007
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负责人:THOMAS F WEISS
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依托单位:
MAGNETIC ALIGNMENT OF BINARY MIXTURES OF PHOSPHOLIPIDS
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批准号:7598308
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项目类别:
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资助金额:$0.22万
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财政年份:2007
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负责人:THOMAS F WEISS
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依托单位: